Effect and mechanism of paeoniflorin on LPS-induced bronchial epithelial cell inflammatory response based on NF-κB pathway
10.3969/j.issn.1000-484X.2024.07.007
- VernacularTitle:基于NF-κB通路研究芍药苷对LPS诱导支气管上皮细胞炎症反应的作用及机制研究
- Author:
Fang HE
1
;
Min DING
;
Haining ZHEN
;
Yajun CHEN
;
Xinxin XUE
Author Information
1. 武汉市第三医院呼吸与危重症医学科,武汉 430060
- Keywords:
Paeoniflorin;
Asthma;
Inflammatory response;
Bronchial epithelial cells;
NF-κB pathway
- From:
Chinese Journal of Immunology
2024;40(7):1387-1391
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of paeoniflorin on LPS-induced bronchial epithelial cell inflammatory response by regulating NF-κB pathway.Methods:BEAS-2B cells were cultured in vitro for paeoniflorin toxicity assay and concentration screening.BEAS-2B cells were divided into control group,LPS group,LPS+CAPE group,LPS+PF group and LPS+CAPE+PF group.Inflammatory responses were induced in BEAS-2B cells using LPS(1 μg/ml),and cell viability was detected by CCK-8 assay after administration of paeoniflorin or CAPE interventions.Apoptosis and cell cycle were detected by flow cytometry.The levels of IFN-γ,IL-4,IL-17C and IL-10 were detected by ELISA.The protein expression levels of p53,Bcl-2,Bax,Cyclin1,NF-κB and p-p65 were detected by Western blot.Results:Paeoniflorin increased cell viability,inhibited apoptosis,increased IFN-γ and IL-10 levels(P<0.01),de-creased IL-4 and IL-17C levels(P<0.01),down-regulated the protein expression levels of p53,Bax,NF-κB and p-p65(P<0.01),and up-regulated the protein expression levels of Bcl-2 and Cyclin1(P<0.01).The effect of paeoniflorin was more significant after the intervention of NF-κB inhibitor CAPE.Conclusion:Paeoniflorin reduces LPS-induced inflammatory factor levels in bronchial epithelial cells by regulating the NF-κB pathway,thereby suppressing the asthmatic inflammatory response.
