Role and mechanism of IL-39 in K562 cells
10.3969/j.issn.1000-484X.2024.07.002
- VernacularTitle:IL-39在K562细胞中的作用及机制研究
- Author:
Kangkang LYU
1
;
Mingzhu XU
;
Bo HU
;
Yuejun LIU
Author Information
1. 苏州大学附属第一医院,苏州 215006
- Keywords:
IL-12;
IL-39;
K562 cells;
Leukemia
- From:
Chinese Journal of Immunology
2024;40(7):1355-1358
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role and mechanism of IL-39 in K562 cells.Methods:The expressions of IL-39R and STAT1/STAT3 in K562 cells were detected via qRT-PCR;the protein levels of phospho-STAT1/STAT3 and STAT1/STAT3 in K562 cells were detected by Western blot;the mRNA regulated by IL-39 in K562 cells was predicted by RNA sequencing.Results:rIL-39 significantly promoted the expression of IL-39R in K562 cells;IL-39 affected K562 cells through STAT1 pathway;IL-39 significantly regulated the expression of mRNA in K562 cells,thus potentially affecting a series of biological processes.Conclusion:IL-39 can directly act on K562 cells,alter gene transcriptional expression and inhibit tumor growth through the STAT1 pathway.IL-39 may similarly modulate gene expression profile in human leukemia cells.
