Circular RNA Circ_0000291/miR-326/PTBP1 axis affect progression of hepatocellular carcinoma
10.3969/j.issn.1000-484X.2024.06.016
- VernacularTitle:环状RNA Circ_0000291/miR-326/PTBP1轴影响肝细胞癌进展
- Author:
Min PANG
1
;
Fang DENG
;
Shanshan LI
Author Information
1. 山东国欣颐养集团枣庄中心医院检验科,枣庄 277000
- Keywords:
Circ_0000291;
miR-326;
PTBP1;
Hepatocellular carcinoma;
Proliferation;
Apoptosis;
Immune escape
- From:
Chinese Journal of Immunology
2024;40(6):1210-1216
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Circ_0000291 on proliferation,apoptosis and immune escape profiling of he-patocellular carcinoma(HCC)cells as a molecular sponge to regulate miR-326 through targeting polypyrimidine tract-binding protein 1(PTBP1).Methods:The expressions of Circ_0000291,miR-326 and PTBP1 in tissue samples and cells were measured by qRT-PCR.Dual luciferase reporter assay was employed to confirm the targeting relationship between Circ_0000291 and miR-326 as well as miR-326 and PTBP1.CCK-8 assay,Transwell assay,flow cytometry assay and ELISA were respectively utilized to determine the prolifera-tion,invasion,apoptosis and the expressions of immune regulation related factors(TNF-α,IFN-γ).Results:Compared with normal tissue and hepatic cells,Circ_0000291 and PTBP1 expressions were overexpressed in HCC tissue and cells,but the expression of miR-326 was obviously down-regulated(all P<0.05).Moreover,functional assays indicated that downregulation of Circ_0000291 could inhibit the proliferation,invasion,and immune escape while induce apoptosis of HCC cells(all P<0.05),which was according-ly exhibited in miR-326 transfection group.Overexpression of Circ_0000291 partly attenuated the anti-tumor effects induced by miR-326(all P<0.05).In HCC,Circ_0000291 could promote the expression of PTBP1 as a molecular sponge to regulate miR-326.Conclu-sion:Circ_0000291 served as a molecular sponge of miR-326 to increase PTBP1 expression and promote the proliferation,invasion and immune escape but inhibit apoptosis of HCC.There upon then,it facilitates the progression of HCC.
