Effect of neferine on blood-brain barrier in rats with ischemic stroke by regulating CCL2-CCR2 signal pathway
10.3969/j.issn.1000-484X.2024.06.009
- VernacularTitle:甲基莲心碱通过调节CCL2-CCR2信号通路对缺血性脑卒中大鼠血脑屏障的影响
- Author:
Chunxiao ZHAI
1
;
Ping TIAN
;
Hao LI
;
Bei MENG
Author Information
1. 信阳职业技术学院护理学院,信阳 464000
- Keywords:
Neferine;
CC chemokine ligand 2-CC chemokine receptor 2 signal pathway;
Ischemic stroke;
Blood-brain barrier
- From:
Chinese Journal of Immunology
2024;40(6):1166-1171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of neferine(NEF)on the blood-brain barrier in ischemic stroke(IS)rats by regulating the CC chemokine ligand 2(CCL2)-CC chemokine receptor 2(CCR2)signal pathway.Methods:SD rats were randomly divided into model group,low-dose NEF group(NEF-L group,25 mg/kg NEF),high-dose NEF group(NEF-H group,50 mg/kg NEF),and high-dose NEF+CCR2 antagonist group(NEF-H+RS102895 group,50 mg/kg NEF+10 mg/kg RS102895),sham opera-tion group(Sham group),with 15 rats in each group.The neurological function scores of rats in each group were compared.The perme-ability of blood-brain barrier of rats in each group was evaluated by Evans blue(EB)permeation method.The water content of brain tis-sue of rats in each group was determined by dry and wet weight method.The expression levels of CCL2 and CCR2 mRNA in rat brain tissue were measured by real-time fluorescence quantitative PCR(RT-qPCR).The expression levels of CCL2,CCR2,ZO-1 and Clau-din-5 proteins in rat brain tissue were detected by Western blot.Results:Compared with Sham group,the neural function score,EB content,water content,the expression levels of CCL2,CCR2 mRNA and protein in the right brain tissue of the model group were obvi-ously higher(P<0.05),the expression levels of ZO-1 and Claudin-5 were lower(P<0.05).Compared with the model group,the neuro-logical function score,water content,EB content,the expression levels of CCL2,CCR2 mRNA and protein in the right brain tissue of rats in the NEF-H group were obviously lower(P<0.05),the expression levels of ZO-1 and Claudin-5 were obviously higher(P<0.05).RS102895 enhanced the protective effect of NEF on the blood-brain barrier in IS rats.Conclusion:NEF may protect the blood-brain barrier of IS rats by inhibiting CCL2-CCR2 signal pathway.
