lncRNA SNHG16 regulates proliferation and invasion of rheumatoid arthritis fibroblast-like synoviocytes through miR-182-5p/PPARG axis
10.3969/j.issn.1000-484X.2024.06.006
- VernacularTitle:lncRNA SNHG16通过miR-182-5p/PPARG轴调控类风湿关节炎成纤维样滑膜细胞增殖和侵袭
- Author:
Wenyu ZHOU
1
;
Wenli CHEN
;
Wenyuan GAN
;
Jiangman SHEN
Author Information
1. 武汉市第四医院,武汉 430030
- Keywords:
Rheumatoid arthritis;
Fibroblast-like synoviocytes;
lncRNA SNHG16;
miR-182-5p;
PPARG
- From:
Chinese Journal of Immunology
2024;40(6):1147-1152
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the expression of small nucleolar RNA host gene 16(SNHG16)in fibroblast-like synovio-cytes(FLS)of patients with rheumatoid arthritis(RA)and its role in the development of RA.Methods:RT-qPCR was performed to measure SNHG16,miR-182-5p and peroxisome proliferator-activated receptor gamma(PPARG)mRNA expression;dual-luciferase assay was performed to measure the interaction between SNHG16,miR-182-5p and PPARG mRNA;EdU and Transwell were per-formed to measure cell proliferation and invasion;Western blot was performed to measure PPARG protein level.Results:SNHG16 and PPARG mRNA expression were up-regulated and miR-182-5p expression was down-regulated in RA synovial tissue and human RA-FLS line(HFLS-RA).SNHG16 negatively targeted miR-182-5p expression and positively regulated PPARG(miR-182-5p target)expression.Silencing of SNHG16 inhibited the proliferation and invasion of HFLS-RA;down-regulation of miR-182-5p partially re-versed the inhibitory effect of SNHG16 silencing on cell proliferation and invasion;overexpression of PPARG partially reversed the in-hibitory effect of up-regulation of miR-182-5p on the proliferation and invasion of HFLS-RA.Conclusion:Silencing SNHG16 target-ing miR-182-5p/PPARG axis inhibits the proliferation and invasion of HFLS-RA.
