Feature gene mining for prediction of acute exacerbation of childhood asthma
10.3969/j.issn.1000-484X.2024.06.002
- VernacularTitle:儿童哮喘急性发作的特征基因挖掘及其对哮喘发作的预测前景
- Author:
Yuyi HUANG
1
;
Junyan ZHANG
Author Information
1. 广州医科大学附属第二医院广东省过敏反应与免疫重点实验室,广州 510260
- Keywords:
Acute asthma;
Gene;
Regulatory network;
Bioinformatics
- From:
Chinese Journal of Immunology
2024;40(6):1126-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Analyzing the gene expression microarray of patients experiencing acute asthma by bioinformatics methods,in order to explore the mechanism of acute asthma and to identify early indicator of acute asthma attacks.Methods:The GSE103166 microarray dataset was retrieved from the GEO database,and after cleaning and organizing the data,differential expres-sion analysis was conducted between children experiencing acute asthma attacks and non-asthmatic children.Enrichment analysis was performed using R language to annotate the functions of the differentially expressed genes(DEGs).Using gene interaction informa-tion,a network of interactions among these genes was constructed,and the network structure was deconstructed to identify key genes and regulatory sub-networks.Results:GSE103166 data set contained 87 nasal swab specimens from children(56 cases of acute asth-ma,31 cases of control).Compared with control group,the acute asthma group had 63 differentially expressed genes(39 upregulated and 24 downregulated).The results of enrichment analysis showed that the DEGs were primarily enriched in endocytic vesicle and en-docytic vesicle membrane.Among these,CD163 was the only gene significantly upregulated in both cellular components.The network dismantling analysis demonstrated four different sub-networks.CD163 was involved in sub-networkⅠ,which was composed of CD163,ARG2,CAT,GSTA2,SCNN1G and MT2A.Among them,ARG2 and CAT were at the regulatory center of the network.Conclusion:CD163 and regulatory networks based on CD163,ARG2,CAT,GSTA2,SCNN1G and MT2A are possible the crucial factors contributing to acute asthma.This study provides research ideas for the precise and early identification of biomarkers and thera-peutic targets for acute asthma attacks.
