Characteristics of immune repertoire of SARS-CoV-2 patients in different infected stages:An analysis based on single cell TCR sequencing
10.3969/j.issn.1000-484X.2024.05.029
- VernacularTitle:单细胞TCR测序分析SARS-CoV-2感染患者不同感染时期免疫组库特征
- Author:
Ke NING
1
;
Jianlong GAO
;
Enze MA
;
Xiao ZHU
Author Information
1. 哈尔滨师范大学计算机科学与信息工程学院,哈尔滨 150025
- Keywords:
TCR;
Immune repertoire;
Infected stages;
Global characteristics
- From:
Chinese Journal of Immunology
2024;40(5):1096-1101
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Using single cell sequencing to analyze the characteristics of the immune repertoire of SARS-CoV-2 pa-tients at different infection stages,and to explore the possible pathogenesis.Methods:Obtaining data in the NCBI database,and healthy control,progression and convalescence groups were set up.Analysis of single cell sequencing data by RStudio,Origin,Hipliot and Excel software.Results:TCR plays an important role in antigen recognition and virus clearance.Characteristics of the three immune repertoires are very different.The number of clones exceeded 26.92%.The length distribution of α chain CDR3 was con-centrated on 14 amino acids,while β-chain was concentrated on 15 amino acids.The frequency of gene fragments in V and J regions were different,and the frequency of TRAV13-1,TRAJ20,TRBV20-1 and TRBJ2-1 were statistically significant(P<0.05).Further V-J gene combination analysis showed that there were significant differences in the frequency of single chain V-J between the control group and the progression group,the control group and the rehabilitation group(P<0.05).The highest frequency of αβ double chain V-J in the control group was TRAV19-J34-TRBV4-1-J2-1,the highest frequency of αβ double-stranded V-J in the progression group was TRAV12-1-J30-TRBV19-1-J2-1,and the highest frequency of αβ double chain V-J in the convalescence group was TRAV12-2-J52-TRBV7-9-J1-5.Conclusion:This study analyzes the global characteristics of T cells in the immune repertoire,which is helpful to understand the pathogenesis of SARS-CoV-2 patients,timely and effective treatment of patients in the early stages of infection.
