Exploring mechanism of Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction in treatment of post-stroke depression based on network pharmacology,molecular docking and animal experiment
10.3969/j.issn.1000-484X.2024.05.027
- VernacularTitle:基于网络药理学、分子对接和动物实验探究柴胡龙骨牡蛎合甘麦大枣汤治疗脑卒中后抑郁的作用机制
- Author:
Hongmei MA
1
;
Jiaming LIU
;
Qiqi CHEN
;
Zhenyu ZHANG
;
Zhiqiang HUANG
;
Yong CHEN
;
Hongfeng LEI
;
Xinju HOU
Author Information
1. 南昌市洪都中医院,南昌 330000
- Keywords:
Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction;
Post-stroke depression;
Network pharmacology;
Molecular docking;
Animal experiment
- From:
Chinese Journal of Immunology
2024;40(5):1082-1088,1095
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore mechanism of Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction in treatment of post-stroke depression(PSD)based on network pharmacology,molecular docking and animal experiment.Methods:TCMSP and other databases were used to predict active components and targets of Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction.Targets of PSD were retrieved from PharmGKB and other databases,and"component-intersection target-disease"network was constructed by Cytoscape(v3.9.1)software.PPI network was constructed by String(v11.5)database,and GO enrichment and KEGG pathway analy-sis of intersection targets were performed by DAVID6.8 database.AutoDock vina(v1.1.2)software was used for molecular docking.Pymol(v 2.5)and other softwares were used to visualize optimal docking results.Animal experiments were setup in control group,model group,fluoxetine group,TCM group and TCM+fluoxetine group,neurobehavioral scores and expressions of neurotransmitters and inflammatory factors in brain tissues were detected.mRNA and protein expressions of key genes PPARG,MAPK3,AKT1,PIK3CA were detected by RT-qPCR and Western blot.Results:A total of 225 kinds of active ingredients of Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction were obtained,which acted on 119 targets of PSD,among which key targets included MAPK3,AKT1,PIK3CA and PPARG,key pathways including MAPK signaling pathway,PI3K-Akt signaling pathway and etc.Compared with model group,MAPK3 mRNA and protein expressions were decreased,AKT1,PIK3CA,PPARG mRNA and protein expressions were increased in TCM group and TCM+fluoxetine group(P<0.05).Conclusion:Mechanism of Chaihu Longgu Muli Decoction and Ganmai Dazao Decoction in treatment of PSD may be related to inhibition of MAPK3 expression,promotion of AKT1,PIK3CA,PPARG expressions,alleviation of inflammatory response and oxidative stress in brain tissues.
