Analysis of effect of moxibustion intervention on cytotoxic T-lymphocyte antigen-4 in immunosuppressed rabbits
10.3969/j.issn.1000-484X.2024.05.024
- VernacularTitle:艾灸干预免疫抑制兔细胞毒性T淋巴细胞抗原-4的作用效应分析
- Author:
Luojie XIONG
1
,
2
;
Yuefeng TIAN
;
Xiaoshan XU
;
Chuntao ZHAI
;
Wei LI
Author Information
1. 山西中医药大学第二临床学院,晋中 030619
2. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Keywords:
CTLA-4;
Herbal cake-partitioned moxibustion;
Immunosuppression;
Cyclophosphamide;
PD-1
- From:
Chinese Journal of Immunology
2024;40(5):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare similarities and differences in expression changes of cytotoxic T-lymphocyte antigen-4(CT-LA-4)and programmed death receptor 1(PD-1)in immunosuppressed rabbits under different moxibustion interventions.Methods:Twenty large-eared white rabbits were randomly divided into normal group,model group,moxa stick moxibustion(MSM)group and herbal cake-partitioned moxibustion(HPM)group,with five rabbits in each group.CTX-induced immunosuppressed models were prepared by intraperitoneal injection for 7 consecutive days.After successful modeling,MSM and HPM were performed on alternate days for 10 treatments.Rabbits were anesthetized after treatment,and serum,liver and spleen were collected.Serum PD-1 and PD-L1 contents were detected by ELISA.PD-1 in liver tissue was detected by immunohistochemistry,and CTLA-4 mRNA in liver and spleen tissues were detected by RT-qPCR.Results:Both HPM and MSM could reduce PD-1 and PD-L1 levels due to immunosuppression,and could effectively suppress elevated levels of CTLA-4 in spleen and PD-1 and CTLA-4 in liver,which were statistically different compared with immunosuppression model group(P<0.05).Pearson correlation test showed that CTLA-4 and PD-1 in liver tissue had significant positive correlation(r=0.780 7,P<0.001).Conclusion:HPM can improve body immune function by regulating multiple immunosuppressive sites.
