Application of network pharmacology and experimental validation in investigating therapeutic potential of puerarin for ulcerative colitis
10.3969/j.issn.1000-484X.2024.05.023
- VernacularTitle:基于网络药理学及实验验证探究葛根素治疗溃疡性结肠炎的作用机制
- Author:
Wenli DAN
1
,
2
;
Xin ZHAO
;
Xingyu LU
;
Zichan GUO
;
Qi QIN
;
Juan LI
;
Kang TANG
;
Huiyuan ZHANG
;
Jinghong SHI
;
Lihua CHEN
Author Information
1. 陕西中医药大学医学技术学院,咸阳 712046
2. 空军军医大学基础医学院,西安 710032
- Keywords:
Puerarin;
Ulcerative colitis;
Network pharmacology;
Epithelial cell apoptosis
- From:
Chinese Journal of Immunology
2024;40(5):1055-1063
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore therapeutic efficacy and mechanism of puerarin(PUE)in treating of ulcerative colitis(UC).Methods:Network pharmacology and molecular docking technique were used to screen and analyze targets of PUE in regulating UC.C57BL/6 mice were given free access to 2.5%DSS aqueous solution for 7 days,and influence of PUE on changes in body weight and disease activity index(DAI)score were subsequently observed.Histopathological alterations of colon tissue were observed by HE staining,changes of goblet cell population in colon tissue were evaluated through Alcian blue staining;expressions of inflammatory factors in colon tissue were detected by qRT-PCR and ELISA.Effect of PUE on MODE-K cell viability and apoptosis were assessed by CCK-8 and flow cytometry.Results:A total of 38 common targets of PUE in modulating UC,such as AKT1,TNF,STAT3,CASP3,HIF1A and etc,mainly involving TNF,IL-17 and PI3K-Akt signaling pathway.In vivo experiments confirmed that PUE ameliorated degree of colon shortening,body weight and DAI scores and reduced inflammatory cell infiltration in mice.Besides,expressions of inflammatory factors in colon,such as TNF-α and IL-1β,were inhibited by PUE.Furthermore,in vitro experiments validated that PUE relieved DSS-induced apoptosis of epithelial cells.Conclusion:PUE alleviates occurrence and development of DSS-induced UC in mice.
