Effects of sinomenine on LPS-induced apoptosis and autophagy of lung epithelial cells in JNK/c-Jun signaling pathway
10.3969/j.issn.1000-484X.2024.04.010
- VernacularTitle:青藤碱在JNK/c-Jun信号通路中对LPS诱导的肺上皮细胞凋亡和自噬的影响
- Author:
Li LI
1
;
Yingying SUN
;
Ying BAI
;
Luowen HU
;
Qingqing WEI
;
Yupeng YAN
;
Ji WANG
Author Information
1. 应急总医院重症医学科,北京 100028
- Keywords:
Sinomenine;
JNK/c-Jun signaling pathway;
LPS;
Lung epithelial cells;
Apoptosis;
Autophagy
- From:
Chinese Journal of Immunology
2024;40(4):731-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of sinomenine(SIN)on LPS-induced apoptosis and autophagy of lung epithelial cells through the JNK/c-Jun signaling pathway.Methods:MLE-12 lung epithelial cells were cultured,and the toxicity of SIN was detected by CCK-8.Apoptosis was detected by flow cytometry,the number of autophagosomes was detected by immunofluorescence,and the expression levels of apoptosis,autophagy and JNK/c-Jun signaling pathway-related proteins were detected by Western blot.Results:After LPS modeling,apoptosis rate and the number of autophagosomes were increased,the protein levels of Cleaved caspase-3,Bax,and Beclin-1,and LC3Ⅱ/LC3Ⅰ,p-JNK/JNK and p-c-Jun/c-Jun were increased(P<0.05);Bcl-2 and P62 protein levels were decreased(P<0.05).SIN treatment can significantly improve the effects of LPS on apoptosis and autophagy,as well as the regulation of the JNK/c-Jun signaling pathway(P<0.05).Treatment with the autophagy inhibitor 3-MA or the JNK agonist ANISO could partially reverse the protective effect of SIN on LPS-induced lung epithelial cells(P<0.05).Conclusion:SIN may increase autophagy and pro-tect lung epithelial cells damaged by LPS by regulating proteins related to the JNK/c-Jun signaling pathway.
