Exploring the effect and mechanism of α-Linolenic acid on neuroin-flammation based on network pharmacology and in vitro experi-ments
10.12092/j.issn.1009-2501.2024.10.004
- VernacularTitle:基于网络药理学和体外实验探究α-亚麻酸改善神经炎症作用及机制
- Author:
Tao ZHANG
1
;
Ruowei WANG
;
Jialin FU
;
Yue GAO
;
Mingyuan HU
;
Zhengmei FANG
;
Yan CHEN
;
Yingshui YAO
Author Information
1. 皖南医学院公共卫生学院慢性病防制研究所,芜湖 241002,安徽
- Keywords:
α-Linolenic acid;
network pharmacol-ogy;
neuroinflammation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(10):1110-1119
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the core target and mechanism of α-Linolenic acid(ALA)in improving neuroinflammation through network pharmacology combined with in vitro experiments.METHODS:Pharmacological studies have shown that ALA has anti-inflammatory,antioxidant,and neuroprotec-tive properties.The targets of α-Linolenic acid were obtained from PharmMapper and Swiss Tar-get Prediction databases,the targets of neuroin-flammation were searched from GeneCards,TTD and OMIM databases,and the potential targets of ALA and neuroinflammation were obtained from Wayne diagram.Protein interaction network(pro-tein-protein interaction,PPI)of potential targets was constructed by STRING website,and the core targets in PPI were screened by Cytoscape 3.8.0 software.At the same time,potential targets are imported into DAVID database,GO and KEGG data were obtained and the results were visualized.Autodock vina and Pymol software were used to dock the selected core targets with ALA and visual-ize the results.An in vitro model of neuroinflamma-tion was constructed,and cell growth status,oxida-tive stress,and migration or repairing capacity were determined by CCK-8 analysis,SOD,MDA and cell scratches,and the expression of IL-6,iba 1,COX-2(PTGS2),and iNOS proteins was determined by ELISA or Western blot experiments.RESULTS:Network pharmacology analysis revealed 46 poten-tial targets of ALA for neuroinflammation,and 10 core targets,including IL-6 and PTGS 2.With 232 entries enriched by GO enrichment analysis and 70 signaling pathways enriched by KEGG enrichment analysis,molecular docking showed that ALA can form hydrogen bonding with COX-2.Experiments showed that ALA could improve cell viability,allevi-ate cell oxidative stress levels,and promote cell mi-gration and motor repair in an in vitro model of neuroinflammation.CONCLUSIONS:ALA may im-prove neuroinflammation by alleviating oxidative stress and inhibiting IL-6 and COX-2 protein expres-sion.
