Eriodictyol resists vascular remodeling in spontaneously hyperten-sive rats by inhibiting TLR4/NF-κB signaling
10.12092/j.issn.1009-2501.2024.09.005
- VernacularTitle:圣草酚改善自发性高血压大鼠血管重构的机制研究
- Author:
Huan WANG
1
;
Junxiu ZHANG
;
Ya ZHANG
;
Hao RONG
;
Youdi WANG
;
Wusan WANG
;
Tongjun MA
Author Information
1. 皖南医学院药学院,芜湖 241002,安徽
- Keywords:
hypertension;
vascular remodeling;
inflammation;
TLR4;
NF-κB
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(9):1002-1010
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the vascular remodel-ing of eriodictyol(EDT)in spontaneously hyperten-sive rats(SHRs)by inhibiting TLR4/NF-kB signaling and to investigate the potential mechanism of ac-tion.METHODS:WKY normal control,SHRs model and EDT administration(EDT 120 mg/kg,SHRs+EDT)group were set for 20 weeks.Tail cuff method for blood BP measurement(SBP,DBP and MBP).Ul-trasonic detection of pulse wave velocity(PWV).The aortic media membrane thickness(MT)was vi-sualized by HE staining.The percentage of aortic collagen(VFC)changes were observed by MASSON staining,Serum content of TNF-α,IL-6,and IL-10 was measured by ELISA.The TNF-α,IL-6,and IL-10 mRNA changes in the aorta were detected by q-PCR.The aortic Collagen Ⅰ and Collagen Ⅲ expres-sion was observed by immunohistochemistry,WB measured the expression of aortic TGF-β1,MMP-2,MMP-9,TLR4,p-IκBa,IκBa,p-p65 and p65.RE-SULTS:After 20 weeks of EDT administration,SBP,DBP,MBP and PWV of SHRs were significantly de-creased,MT and VFC of aorta were significantly de-creased,and protein expressions of Collagen Ⅰ,Col-lagen Ⅲ,TGF-β1,MMP-2,MMP-9,TLR4,p-l Camba and p-p65 were significantly decreased.CONCLU-SION:After long-term administration of EDT could inhibit TLR4/NF-κB signaling and exert anti-inflam-matory effects,thus reducing TGF-β1,MMP-2 and MMP-9 expression,decreasing collagen content,and finally improving aortic remodeling and sclero-sis of SHRs.
