Mechanism of PX-12 induced apoptosis of hepatocellular carcinoma cells through oxidative stress
10.12092/j.issn.1009-2501.2024.09.001
- VernacularTitle:PX-12通过氧化应激诱导肝癌细胞凋亡的作用机制
- Author:
Guojie LEI
1
,
2
;
Yanhua YU
;
Yingchao LIU
;
Wenxia BIAN
;
Jing DU
;
Xiangmin TONG
Author Information
1. 浙江中医药大学,杭州 310053,浙江
2. 浙江省人民医院(附属人民医院),杭州医学院,检验中心,杭州 310006,浙江
- Keywords:
hepatocellular carcinoma;
PX-12;
oxi-dative stress;
apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(9):961-967
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the mechanism of PX-12 induced apoptosis of hepatocellular carcinoma cells.METHODS:Human hepatoma cell line Huh7 was se-lected as the main research object.After the cells were treated with thioredoxin inhibitor PX-12,the cell viability was detected by CCK8 method,the cell mi-gration ability was detected by cell scratch test,the cell proliferation ability was detected by cell prolif-eration kit,the levels of intracellular reactive oxygen species and apoptosis were detected by flow cy-tometry,and the expression of apoptosis-related proteins were detected by Western blot.RESULTS:Compared with the control group,the cell viability,migration ability and proliferation ability of PX-12 treatment group were significantly decreased(P＜0.05),and the level of intracellular reactive oxygen species was increased(P＜0.05)in a concentration-dependent manner.Apoptosis inhibitor Z-VAD-FMK and antioxidant NAC could restore the cell viability,and NAC could reduce the accumulation of intracel-lular reactive oxygen species induced by PX-12 and restore the apoptosis induced by PX-12(P＜0.05).CONCLUSION:PX-12 induces apoptosis of hepato-cellular carcinoma cells through oxidative stress.
