Caragana sinica root inhibits Erastin-induced chondrocyte ferroptosis by blocking TRPM7
10.12092/j.issn.1009-2501.2024.07.010
- VernacularTitle:金雀根通过阻断TRPM7抑制Erastin诱导的软骨细胞铁死亡
- Author:
Rendi ZHU
1
,
2
;
Biao QU
;
Renpeng ZHOU
;
Wei HU
Author Information
1. 安徽医科大学药学院,合肥 230032,安徽
2. 安徽医科大学第二附属医院药物临床试验研究中心,合肥 230601,安徽
- Keywords:
Caragana sinica root;
chondrocytes;
Erastin;
ferroptosis;
TRPM7
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(7):800-808
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Cara-gana sinica root(CSR)on ferroptosis in the Erastin-induced chondrocyte ferroptosis model and possi-ble mechanisms.METHODS:The C28/I2 chondro-cyte cell line was cultured to construct a cell model of Erastin-induced ferroptosis.Cell viability was de-tected by MTT assay;cell death was observed by Calcein/PI staining;cell lactate dehydrogenase(LDH)and total glutathione(GSH)levels were de-tected by the kit;reactive oxygen species(ROS)lev-els were detected by fluorescent probe BODIPY 581/591 C11 labeling;mitochondrial membrane po-tential(ΔΨm)changes were observed by Rh123 and JC-1 staining;Western blot was used to detect the expression of ferroptosis-related proteins(AC-SL4,GPX4)and TRPM7 proteins.RESULTS:Erastin treatment decreased chondrocyte viability,in-creased cytotoxicity,induced oxidative stress,dis-rupted ΔΨm,and up-regulated ACSL4 protein ex-pression,while down-regulating GPX4 protein ex-pression and inducing chondrocyte ferroptosis.In contrast,CSR restored cell viability and reduced oxi-dative stress,thereby inhibiting chondrocyte fer-roptosis.In addition,CSR reduced the Erastin-in-duced increase in TRPM7 protein expression level.CONCLUSION:Erastin induced lipid peroxidation in C28/I2 chondrocytes,causing mitochondrial dam-age and ferroptosis;CSR may inhibit chondrocyte ferroptosis by blocking TRPM7,thus exerting a pro-tective effect on chondrocytes.
