Comprehensive protein kinase inhibition analysis reveals the molecu-lar mechanism of KG-1 proliferation
10.12092/j.issn.1009-2501.2024.06.003
- VernacularTitle:蛋白激酶全抑制分析揭示KG-1细胞增殖的分子机制
- Author:
Yu DUAN
1
;
Ningxin XU
;
Qiong CAO
;
Kai YANG
;
Jinjuan WANG
;
Sijin LIU
;
Fengfeng JIA
;
Jianbing LIU
;
Li LI
Author Information
1. 山西医科大学基础医学院医学细胞生物与遗传学教研室,太原 030001,山西
- Keywords:
KG-1 cell line;
protein kinase inhibi-tors;
FGFR1OP2-FGFR1fusion gene;
STAT5
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(6):621-628
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the molecular mechanisms of KG-1 cell proliferation by profiling its responses to various protein kinase inhibitors.METHODS:CCK-8 assay,real time quantitative PCR(qRT-PCR)and Western-blot were used to detect the effect of various protein kinase inhibitors on KG-1 cell proliferation,the expression levels of mRNA and phosphorylation level of signaling pro-teins in the FGFR1 downstream pathways.RE-SULTS:NVP-BGJ398 and PD173074 effectively in-hibited the proliferation of KG-1 cells,indicative of a crucial role of FGFR downstream signaling.After treatment with FGFR inhibitors,the levels of p-FG-FR1OP2-FGFR1 and p-STAT5 decreased significantly(P<0.001),p-AKT decreased slightly(P<0.05),with-out affecting the p-ERK level(P>0.05).CONCLU-SION:FGFR1OP2-FGFR1 mainly acts on the down-stream STAT5 signaling pathway to promote cell proliferation.Comprehensive protein kinase inhibi-tion analysis is a reliable and direct approach to identify functional drivers of cancer cell prolifera-tion.
