Screening and biologically functional analysis of differentially expressed genes for neuropathic pain
10.3969/j.issn.1001-1242.2024.10.004
- VernacularTitle:神经病理性疼痛差异表达基因的筛选及生物学功能分析
- Author:
Peng SHEN
1
;
Xinping CHAI
;
Yutong LI
Author Information
1. 南昌大学第一附属医院康复医学科,江西省南昌市,330000
- Keywords:
neuropathic pain;
differentially expressed genes;
bioinformatics
- From:
Chinese Journal of Rehabilitation Medicine
2024;39(10):1430-1435,1442
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate differentially expressed genes(DEGs)for neuropathic pain between sciatica and healthy controls. Method:The microarray dataset GSE150408 were downloaded from gene expression database(gene expression omnibus database,GEO).The DEGs were screened using the limma V3.42.0(linear models for microarray da-ta)package of the R software program(version 3.5.0).GEO terms and Kyoto encyclopedia of genes and ge-nomes(KEGG)pathway enrichment analysis of DEGs were automatically completed and visualized by the clus-terProiler V3.14.0.STRING was searched to identify and predict interactions between genes or proteins,to con-struct the protein to protein interaction. Result:A total of 424 DEGs were screened,including 233 up-regulated genes and 191 down-regulated genes.The result of GO enrichment indicated that for biological process,DEGs were significantly enriched in de-fense response,regulation of immune system process,cell activation,leukocyte activation.Regarding cell com-ponents,DEGs were significantly enriched in vesicles,secretory granules,tertiary granule.For molecular func-tion,DEGs were significantly enriched in immune receptor activation,complement receptor activity and MAP kinase phosphatase activity.The result of KEGG pathway-enrichment indicated that DEGs were mainly enriched in transcriptional misregulation in cancer.Moreover,the GSEA analysis indicated that the most significant en-riched gene sets included endocytosis,Epstein-Barr virus infection.PPI analysis were performed to explore the potential function of the DEGs.It showed that the degree of FCGR1A was the highest in up-regulated proteins and the highest in down-regulated proteins.These two proteins were the key nodes. Conclusion:It showed an inflammatory and immune characteristic in peripheral blood for neuropathic pain.AZU1,BPI,FCGR1A and SPTBN2 were the key genes in neuropathic pain.
