Effects of exogenous recombinant Annexin A6 on early plasma membrane injury repair of skeletal mus-cle in mice after eccentric exercise
10.3969/j.issn.1001-1242.2024.10.002
- VernacularTitle:外源性重组膜联蛋白A6对小鼠离心运动后骨骼肌早期质膜损伤修复的影响
- Author:
Yanli HAN
1
,
2
;
Shousheng XU
;
Yue ZHOU
Author Information
1. 北京体育大学,北京市,100084
2. 国家体育总局运动应激适应重点实验室
- Keywords:
eccentric exercise;
recombinant human Annexin A6;
skeletal muscle injury;
plasma membrane repair
- From:
Chinese Journal of Rehabilitation Medicine
2024;39(10):1409-1416
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of exogenous recombinant human Annexin A6(rhANX A6)intervention on early plasma membrane damage and its metabolic characteristics in skeletal muscle in mice with eccentric exercise injury models. Method:Fifty-six C57 male mice,aged 8 weeks,were randomly divided into the quiet control group(group C),exercise group injected with phosphate-buffer saline(group EP)and exercise group injected with rhANX A6(group ER).The exercise groups were further subdivided based on different post-exercise sampling times:EP2,EP6,EP12,and ER2,ER6,ER 12,with 8 mice in each subgroup.Two hours before exercise,the group EP and group ER received intraperitoneal injections with l*PBS buffer(1 x concentration)and rhANX A6(0.8 mg/kg),respectively.ELISA was used to detect the content of ANX A6 in serum creatine kinase(CK),Annexin A6(ANX A6)and skeletal muscle in each group of mice,and Evans Blue dye(EBD)was used to observe the damage of the skeletal muscle plasma membrane of each group of mice. Result:The serum CK activity in all EP subgroups and ER12h were significantly higher than those in the C group(P＜0.01).At the same time point,CK levels in all EP subgroups were significantly higher than in the ER group(P＜0.05).The average fluorescence intensity of EBD-positive regions in all EP and ER subgroup was significantly higher than that in group C(P＜0.01).All EP subgroups had significantly higher intensities than the ER group(P＜0.01).The serum ANX A6 levels in all ER subgroups and EP6h,EP12h were signifi-cantly higher than that in group C(P＜0.01).At 12h time point,ANX 46 levels in the ER group were signifi-cantly higher than in the EP group(P＜0.05).ANX A6 levels in skeletal muscle of all EP and ER subgroups were significantly higher than that in group C(P＜0.05);All ER subgroups had significantly higher than those in the EP group(P＜0.05). Conclusion:Exogenous rhANX A6 injection 2 hours before eccentric exercise in mice can significantly improve the early plasma membrane injury in skeletal muscle after exercise.It is speculated that rhANX A6 directly in-volved in the repair of early plasma membrane damage in skeletal muscle induced by eccentric exercise.
