Mechanism of enriched environment-induced improvement of memory by promoting synaptic remodeling in the CA3 region of the hippocampus in chronic cerebral ischemia rats
10.3969/j.issn.1001-1242.2024.07.003
- VernacularTitle:丰富环境通过促进海马CA3区突触重塑改善慢性脑缺血大鼠记忆减退的机制研究
- Author:
Liangfeng SHI
1
;
Chuanjie WANG
;
Feng TAO
Author Information
1. 复旦大学附属金山医院内分泌科,上海市,201508
- Keywords:
enriched environments;
chronic cerebral ischemia;
synaptic remodeling;
memory impairment
- From:
Chinese Journal of Rehabilitation Medicine
2024;39(7):934-939
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the intrinsic mechanism of enriched environment-induced improvement of memory in rats with chronic cerebral ischemia. Method:Forty-eight healthy male SD rats were randomly divided into chronic cerebral ischemia+standard envi-ronment group(CCH+SE),chronic cerebral ischemia+rich environment group(CCH+EE),and Sham surgery+standard environment group(Sham+SE)with 16 rats in each group.The CCH+SE group and CCH+EE group were given bilateral common carotid artery ligation surgery and a rat model of chronic cerebral ischemia was constructed.The Sham+SE group performed the same procedures as the model group without ligating bilateral common carotid arteries.Three groups of rats were raised in corresponding environments for 21 days,and their spatial learning and memory functions were tested using a water maze test.Protein immunoblotting and immunofluorescence techniques were used to detect changes of BDNF,GAP-43,and SYN proteins expressions in the CA3 region of the rat hippocampus. Result:Compared with the standard environment group,the learning and memory functions of the enriched en-vironment group were significantly improved,with a statistically significant difference(P＜0.05).The expres-sion levels of BDNF,GAP-43,and SYN proteins in the CA3 region of the hippocampus were significantly in-creased,with a statistically significant difference(P＜0.05). Conclusion:Enriched environment intervention can improve learning and memory ability in rats with chronic cerebral ischemia,and its underlying mechanism may be promoting synaptic remodeling in the hippocampal CA3 region.
