Exosomal miR-21 derived from Schwann cells promoting the repair of sciatic nerve injury by targeting SPRY2
10.3969/j.issn.1001-1242.2024.06.002
- VernacularTitle:雪旺细胞来源的外泌体miR-21通过靶向SPRY2促进大鼠坐骨神经损伤后修复的研究
- Author:
Mingyue TIAN
1
;
Yiduo YANG
;
Wanting QIN
Author Information
1. 上海中医药大学中西医结合学院人体解剖教研室,上海市,201203
- Keywords:
Schwann cells;
exosome;
miR-21;
sciatic nerve injury;
functional recovery;
nerve regeneration
- From:
Chinese Journal of Rehabilitation Medicine
2024;39(6):767-774
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and potential mechanism of exosomal miR-21 derived from Schwann cell(SC)in the repairment of sciatic nerve injury(SNI). Method:SC was infected with negative control lentivirus and lentivirus that expression of miR-21,and its exosome was collected and identified respectively.The SNI rat model was established by nerve stump anasto-mosis.After surgery,the rats were randomly divided into the model group(n=10),the SC-derived exosome group(n=10)and the SC-derived exosome group(n=10)that high expression of miR-21.The SC-derived exosomes group and SC-derived exosome group that high expression of miR-21 were treated with local injec-tion of exosome collected in vitro.After 3 weeks,the functional recovery was detected by behavioral experi-ment.The nerve regeneration was evaluated by immunofluorescence staining.RT-qPCR was used to detect the expression of miR-21 in serum exosome and miR-21 and SPRY2 in sciatic nerve.The targeting interaction be-tween miR-21 and SPRY2 was verified by dual-luciferase reporter gene experiment. Result:Compared with the model group,the functional recovery and regeneration of sciatic nerve were signifi-cantly improved in the other two groups.The expression of miR-21 was significantly increased,while the ex-pression of SPRY2 was significantly decreased.The improvement in the SC-derived exosome group with high expression of miR-21 were more significantly than those in the SC-derived exosome group. Conclusion:Exosomal miR-21 derived from SC can significantly promote the repair of sciatic nerve injury by targeting SPRY2.
