Safety of low-intensity focused ultrasound for stimulating rat vagus nerve
10.13929/j.issn.1672-8475.2024.06.010
- VernacularTitle:以低强度聚焦超声刺激大鼠迷走神经的安全性
- Author:
Haifen LIAO
1
,
2
;
Wen MENG
;
Lili NIU
;
Min PAN
Author Information
1. 广州中医药大学深圳医院(福田)超声科,广东 深圳 518034
2. 中国科学院深圳先进技术研究院劳特伯生物医学成像研究中心,广东 深圳 518055
- Keywords:
vagus nerve;
rats;
low-intensity focused ultrasound
- From:
Chinese Journal of Interventional Imaging and Therapy
2024;21(6):358-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the safety of low-intensity focused ultrasound(LIFU)for stimulating rat vagus nerve.Methods Twelve male SD rats were randomly divided into stimulation group A,B,C and sham stimulation(D)group(each n=3).A LIFU stimulation system equipped with a suitable collimator was used to stimulate rat left vagus nerve in A,B,C groups,with acoustic pressure of 1.05,3.87 and 4.25 MPa,respectively,with the same pulse repetition frequency,duty ratio and stimulation mode.For rats in group D,the ultrasonic transducer was positioned on the left side of the neck but no stimulation was performed.The changes of heart rate and local neck temperature were recorded before,during and after LIFU stimulation.After stimulation,neck MR T2WI were acquired for group C and D.Then pathological examination of vagus nerve was performed to observe whether there were abnormal changes after stimulation.Results No significant difference of heart rate 5 min before,during and 5 min after stimulation was found among 4 groups(all P>0.05).The local temperature beneath the transducer of rats in group A,B,C and D ranged from 28.1-30.1℃,28.3-30.3℃,27.9-30.4℃ and 27.8-30.2℃,respectively,and the temperature fluctuation all less than 2.5℃.T2WI showed no obvious abnormality of neck soft tissue in group C nor D,while no significant pathological changes of rat vagus nerve was detected.Conclusion LIFU with proper parameters was safe for stimulating rat vagus nerve.
