Histopathologic Characteristics of Conjunctivochalasis.
10.3341/jkos.2013.54.8.1165
- Author:
Jeong Bum BAE
1
;
Woo Chan PARK
Author Information
1. Department of Ophthalmology, Dong-A University College of Medicine, Busan, Korea. wcpark@dau.ac.kr
- Publication Type:Original Article
- Keywords:
Conjunctivochalasis;
Impression cytology;
Lymphangiectasis
- MeSH:
Antibodies, Monoclonal, Murine-Derived;
Biopsy;
Collagen;
Diagnostic Tests, Routine;
Elastic Tissue;
Eosine Yellowish-(YS);
Eye;
Goblet Cells;
Hematoxylin;
Humans;
Immunohistochemistry;
Lymphangiectasis;
Lymphatic Vessels;
Outpatients;
Prospective Studies
- From:Journal of the Korean Ophthalmological Society
2013;54(8):1165-1174
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In this study we investigated the histopathologic characteristics of conjunctivochalasis and its association with lymphangiectasis using impression cytology and conjunctival excisional biopsy in patients with conjunctivochalasis. METHODS: This prospective study included 14 outpatients diagnosed with conjunctivochalasis, and who had excisional biopsies performed between March 1, 2012 and November 30, 2012. Preoperative slit-lamp examination, dry eye diagnostic test and impression cytology were also performed. For histological analysis, conjunctival sections were stained with hematoxylin and eosin (H&E), Verhoeff-van Gieson (VVG) elastic staining, and monoclonal antibody D2-40, a lymphatic endothelial marker. RESULTS: Tear break-up time and Schirmer's value were decreased in patients with conjunctivochalasis. Impression cytology showed a decrease in goblet cell density and an increase in nucleoplasmic/cytoplasmic ratio. The patients' conjunctival tissues exhibited an infiltration increase of chronic inflammatory cells on H&E stain, and a decrease in collagen density with degeneration of elastic fibers on VVG stain compared to tissues from the control subjects. D2-40 immunohistochemistry revealed the dilated subconjunctival lymphatics in patients with conjunctivochalasis. CONCLUSIONS: This study suggests that the characteristic histopathological features of conjunctivochalasis include dilated lymphatic vessels as well as decreased goblet cell and collagen densities with degeneration of elastic fibers.
