Transcriptomic characteristics analysis of bone from chronic osteomyelitis
10.12200/j.issn.1003-0034.20221149
- VernacularTitle:慢性骨髓炎患者骨组织的转录组特征分析
- Author:
Yang ZHANG
1
;
Yi-Yang LIU
;
Li-Feng SHEN
;
Bing-Yuan LIN
;
Dan SHOU
;
Qiao-Feng GUO
;
Chun ZHANG
Author Information
1. 浙江中医药大学附属第一医院浙江省中医院骨伤研究所,浙江 杭州 310053
- Keywords:
Transcriptomics;
Chronic osteomyelitis;
MAPK pathway;
Osteoclast differentiation pathway
- From:
China Journal of Orthopaedics and Traumatology
2024;37(5):519-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of chronic osteomyelitis and to clarify the role of MAPK signal pathway in the pathogenesis of chronic osteomyelitis,by collecting and analyzing the transcriptional information of bone tissue in patients with chronic osteomyelitis.Methods Four cases of traumatic osteomyelitis in limbs from June 2019 to June 2020 were selected,and the samples of necrotic osteonecrosis from chronic osteomyelitis(necrotic group),and normal bone tissue(control group)were collected.Transcriptome information was collected by Illumina Hiseq Xten high throughput sequencing platform,and the gene expression in bone tissue was calculated by FPKM.The differentially expressed genes were screened by comparing the transcripts of the Necrotic group and control group.Genes were enriched by GO and KEGG.MAP3K7 and NFATC1 were selected as differential targets in the verification experiments,by using rat osteomyelitis animal model and im-munohistochemical analysis.Results A total of 5548 differentially expressed genes were obtained by high throughput sequenc-ing by comparing the necrotic group and control group,including 2701 up-regulated and 2847 down-regulated genes.The genes enriched in MAPK pathway and osteoclast differentiation pathway were screened,the common genes expressed in both MAPK and osteoclast differentiation pathway were(inhibitor of nuclear factor κ subunit Beta,IκBKβ),(mitogen-activated protein ki-nase 7,MAP3K7),(nuclear factor of activated t cells 1,NFATC1)and(nuclear factor Kappa B subunit 2,NFκB2).In rat os-teomyelitis model,MAP3K7 and NFATC1 were highly expressed in bone marrow and injured bone tissue.Conclusion Based on the transcriptome analysis,the MAPK signaling and osteoclast differentiation pathways were closely related to chronic os-teomyelitis,and the key genes IκBKβ,MAP3K7,NFATC1,NFκB2 might be new targets for clinical diagnosis and therapy of chronic osteomyelitis.
