Airway Obstruction after Acute Ozone Exposure in BALB/c Mice Using Barometric Plethysmography.
- Author:
An Soo JANG
1
;
Inseon S CHOI
;
Sun Wook KIM
;
Byung Cheol SONG
;
Chung Ho YEUM
;
Joo Young JUNG
Author Information
1. Department of Internal Medicine, Cheju National University College of Medicine, Jeju, Korea. jas877@chollian.net
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Ozone;
Airway obstruction;
Time
- MeSH:
Airway Obstruction/*etiology/*pathology;
Animals;
Animals, Newborn;
Bronchoalveolar Lavage Fluid/cytology;
Disease Models, Animal;
Female;
Mice;
Mice, Inbred BALB C;
Plethysmography, Whole Body/*methods;
Probability;
Reference Values;
Respiratory Function Tests;
Risk Assessment;
Sensitivity and Specificity;
Statistics, Nonparametric;
Sulfuric Acids/*adverse effects
- From:The Korean Journal of Internal Medicine
2003;18(1):1-5
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Airway responsiveness after acute inhalation of ozone is related to the concentration and duration of ozone exposure. Using barometric whole-body plethysmography and increase in enhanced pause (Penh) as an index of airway obstruction, we measured the response of BALB/c mice to acute ozone inhalation to study the time course change of pulmonary function after ozone exposure. METHODS: Penh was measured before and after exposure to filtered air or 0.12, 0.5, 1, or 2 ppm ozone for 3 hr (n=6/group). In addition, Penh was measured 24, 48 and 72 hr after ozone exposure. Bronchoalveolar lavage (BAL) and histopathologic examinations were performed. RESULTS: The increase in Penh after ozone exposure was significantly higher in the 0.12, 0.5, 1 and 2 ppm groups compared with the control group (all p< 0.01). Increases in Penh 24 hr after ozone exposure were significantly lower than those immediately after acute ozone exposure; however, increases in Penh 72 hr after ozone exposure were significantly higher than those in the control group (each p< 0.01). The proportion of neutrophils in BAL fluid was significantly higher in the group exposed to 2 ppm ozone than in the groups exposed to filtered air or 0.12 ppm ozone (both p< 0.01). CONCLUSION: These results indicate that airway obstruction is induced following ozone exposure in a concentration-dependent manner and persists for at least 72 hr.
