Investigation on the role on perindopril for prevention and treatment of glucocorticoid induced osteoporosis in rab-bits
10.3969/j.issn.1003-0034.2016.01.012
- VernacularTitle:培哚普利对兔糖皮质激素性骨质疏松症干预作用的实验研究
- Author:
Feng ZHOU
1
;
Chun RONG
;
Kai WANG
;
Sheng Chun WANG
;
Tao Yong ZHANG
Author Information
1. 青岛大学附属医院骨科
- Keywords:
Glucocorticoid induced osteoporosis;
Bone metabolism;
Perindopril;
Rabbits
- From:
China Journal of Orthopaedics and Traumatology
2016;(1):52-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of perindopril for prevention and treatment of glucocorticoid induced osteo-porosis (GIOP) in rabbits. Methods:A total of 45 male New Zealand white rabbits (10 months old,weight 3.0 to 3.5 kg) were randomly divided into 3 groups involving normal control group (muscle injection of saline solution,n=15,group NC),model group (muscle injection of dexamethasone,n=15,group GIOP),and treatment group (muscle injection of dexamethasone com-bined with oral perindopril,n=15,group GIOP+ACEI). All rabbits put to death after 12 weeks'treatment. The changes of bone mass and strength were observed and analyzed by bone histomorphology ,biomechanics,metabolic bone related serological in-dexes and mRNA expression. Results:At 12 weeks,the analysis of bone histomorphology and biomechanics results showed that the bone mass and bone strength of group GIOP were significantly lower than that of group NC (P<0.05);after perindopril treat-ment,the bone mass and bone strength of group GIOP+ACEI were higher obviously than that of group GIOP (P<0.05). Mineral-izing surface,mineral apposition rate and serum osteocalcin in group GIOP decreased than group NC; however,osteoclast number,osteoclast surface,eroded surface,and urinary deoxypyridinoline in group GIOP increased than group NC (P<0.05);these changes were inhibited after perindopril treatment (P<0.05). Quantitative RT-PCR revealed that after dexamethasone treatment,the ratio of SOST mRNS expression and RANKL/OPG mRNA expression obviously increased than that of group NC (P<0.05);and Runx2 expression decreased significantly (P<0.05);while the changes of mRNA expression were improved by perindopril treatment. Conclusion:Perindopril can promote bone formation and inhibit bone resorption to deduce glucocorti-coid induced osteoporosis. This study provides a new method for prevention and treatment of GIOP.
