Effect of electro acupuncture combined with olfactory ensheathing cell transplantation on spinal cord injury axonal regeneration and direction
10.3969/j.issn.1003-0034.2015.05.012
- VernacularTitle:电针联合嗅鞘细胞移植对大鼠脊髓损伤神经轴突再生及走向的影响
- Author:
Wang Long TAN
1
;
Bang Zhao QIN
;
Feng ZHU
;
Xue Li YANG
Author Information
1. 陕西中医学院附属医院骨伤科
- Keywords:
Spinal cord injuries;
Electro acupuncture;
Olfactory ensheathing cells;
Neuroaxonal regeneration
- From:
China Journal of Orthopaedics and Traumatology
2015;(5):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the impact and mechanism of electro acupuncture(EA)on olfactory ensheathing cells (OECs)transplantation of spinal cord injury(SCI)axonal regeneration. Methods:In the experiment,72 adult Sprague Daw?ley male rats weighted(220±20)g underwent contusion and transection method to cause the T9 model of spinal cord injury, were randomly divided into four groups involving model group,EA group,OECs group,and EA+OECs group. 5%fluorescein gold(FG)solution of 0.5μl was injected into rats' spinal cord at 4 weeks and 8 weeks after SCI,a series of tests were per?formed including fluorescein gold(FG)retrograde tagging,BBB scores. Results:(1)The BBB scores level among four groups had no differences from the 1st day to the 1st week after the SCI(P>0.05). From the 3rd week after the SCI,the BBB scores level in EA+OECs group were obviously higher than that of other groups(P<0.05).(2)The result of the fluorescein gold (FG)retrograde tagging showed at 4 weeks and 8 weeks after treatment FG positive nerve fibers were observed in SCI region. In EA+OECs group the number of FG positive nerve fibers was more than other three groups,and the fibers were more regular?ly arranged than other three groups. Conclusion:The combination of electro acupuncture and OECs transplantation can recov?er the pathway of nerve conduction and promote nerve fibers regeneration and hind limb function recovery for SCI rat ,and can guide the trend of the axonal regeneration.
