Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway
10.3969/j.issn.1003-0034.2014.06.005
- VernacularTitle:河蚌提取物葡聚糖对软骨细胞Wnt通路的调控作用研究
- Author:
Pu Song WEI
1
;
Fang Dao DING
;
Zong Xue WANG
;
Jian PANG
;
Xin Yu ZHENG
;
Guang Qin XU
;
Long Yue CAO
;
Sheng Hong ZHAN
Author Information
1. 上海中医药大学附属曙光医院骨伤科
- Keywords:
Tissue extracts;
Chondrocytes;
Glucans;
Gene expression regulation
- From:
China Journal of Orthopaedics and Traumatology
2014;(6):461-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt path-way in vitro. Methods:Rat chondrocytes were cultured and differentiated induced with IL-1β (10 ng/ml) in vitro. Chondro-cytes were divided into five groups:IL-1βgroup,IL-1β+IWP-2 (5μM,Wnt pathway inhibitor) group,IL-1β+HBP-A (0.3 mg/ml) group and IL-1β+IWP-2+HBP-A group. Wnt-3a,β-catenin (24 h,48 h,72 h) and MMP-13(72 h) genes expres-sion were detected by Rt-PCR,whileβ-catenin,MMP-13,Sox-9 and coll-Ⅱ (48 h) protein expression were measured by Western-blot. Results:After induction of IL-1β,gene expression of Wnt-3a,β-catenin and MMP-13 were increased,so were the protein expression ofβ-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-Ⅱwere declined. Follow-ing addition of HBP-A,Wnt-3a,β-catenin and MMP-13 were shown as induction of IL-1β,but protein expression of Sox-9 and Coll-Ⅱwere upgraded . Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a ,β-catenin gene andβ-catenin protein expression and highest expression of Sox-9 protein. Conclusion:HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt/β-catenin,but also adjust the expression of Wnt-3a,β-catenin and Sox-9 when combinated with the Wnt inhibitor.
