Experimental research in vitro of TK/GCV system for osteosarcoma MG-63 cell damage
10.3969/j.issn.1003-0034.2014.03.017
- VernacularTitle:TK／GCV系统对骨肉瘤MG-63细胞杀伤的体外实验研究
- Author:
Dong Hua ZHANG
1
;
Zhi LV
;
Yi FENG
;
Li Xiao LIU
;
Ming Hui HOU
Author Information
1. 山西医科大学第二医院骨科
- Keywords:
Osteosarcoma;
Gene therapy;
Thymidine kinase/Ganciclovir (TK/GCV);
Bystander effect
- From:
China Journal of Orthopaedics and Traumatology
2014;(3):240-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the killing effects of the liposome mediated thymidine kinase (TK)/ganciclovir (GCV) system on MG-63 osteosarcoma (OS) cells and its bystander effects. Methods:Liposome mediated TK gene transfected into MG-63 OS cells,the efficiency of transfection was analyzed by flow cytometry and observed under inverted fluorescence mi-croscope. Non-transfected osteosarcoma MG-63 cells were divided into three groups ,in the experimental group 1 transfected TK/GCV cells cultured in solutiona liquid mixture by supernatant by 1/10 ,1/7,1/5,1/2 ratio to original broth;in the experi-mental group 2 transfected cells cultured in solutiona liquid mixture of supernatant filtered through 0.22 μm filter by 1/10,1/7,1/5,1/2 ratio to original broth,in control group the transfection cells cultured in original culture solution. Cell growth inhi-bition rate and osteosarcoma cell sensitivity to TK / GCV system were measured by MTT assay in each group. Results:The TK gene was transfected into MG-63 OS cells successfully by liposome mediated,flow cytometry instrument detection TK gene transfection cell transfection efficiency can reach 75.5%. Six days later the MTT assay showed that in the experimental group 1 inhibition rate of all concentration ratio of the mixed culture fluid were statistically significant as compared with the control group (P<0.05),and in the experimental group 2 that of the 1/10 and 1/7 of concentration ratio of mixed culture medium was not statistically significant as compared with the control group (P>0.05). TK gene transfected MG-63 cells increased with the the GCV concentration,the cell apoptosis rate increased. Conclusion:The experiment demonstrated that the MG-63 OS cells are sensitive to the liposome mediated TK/GCV system and bystander effects are significant.
