Effect of Hydroxy Safflower Yellow A on Glucocorticoid induced bone marrow mesenchymal stem cells osteogenic differentiation
10.3969/j.issn.1003-0034.2014.03.013
- VernacularTitle:羟基红花黄色素A对激素诱导骨髓间充质干细胞成骨分化的影响
- Author:
Tian WAN
1
;
Rui Min WU
;
Xi Zhen QI
Author Information
1. 福建中医药大学骨伤学院
- Keywords:
Femur head necrosis;
Hydroxy Safflower Yellow A;
Bone marrow mesenchymal stem cells;
Osteogenic differentiation
- From:
China Journal of Orthopaedics and Traumatology
2014;(3):224-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Hydroxy Safflower Yellow A (HSYA) on the expression of osteogenic markers,such as alkaline phosphatase,Cbfαl and type I collagen,and explore the mechanism of HSYA in the prevention and treatment of glucocorticoid induced ischemic necrosis of femoral head. Methods:Fifteen healthy and adult New Zealand white rabbits were collected and weighted 0.9 to 1.3 kg. The rabbits were injected abdominally with anesthetic drugs ,then received marrow cavity puncture of tibia and anterior superior iliac spine to get bone marrow blood. Rabbits bone marrow mesenchymal stem cells (BMSCs) were separated from the bone marrow blood,cultured in vitro and passaged. The 3rd generation of BMSCs which had good growth condition were randomly divided into blank group ,model group and HSYA groups with different doses. The BMSCs in model group were treated with high dose of dexamethasone to induce adipogenic differentiation of cells cultured in vitro,and inhibit osteogenic differentiation. The BMSCs in HSYA groups received high dose of dexamethasone and different concentrations of HSYA simultaneously. The blank group received not any special handling. After a week ,the expressions of alkaline phosphatase,Cbfαl and type I collagen mRNA were detected. Results:The alkaline phosphatase activity was signifi-cantly decreased in BMSCs of the model group as compared with the blank group (P<0.01),and the expression of Cbfαl and type I collagen mRNA were also decreased significantly (P<0.01). The alkaline phosphatase activity was significantly in-creased in BMSCs of each HSYA group as compared with the model group (P<0.05 or P<0.01),and the expression of Cbfαl and type I collagen mRNA were also increased significantly (P<0.05 or P<0.01). Conclusion:The mechanism of HSYA may be related to the effect of antagonism to the reduced osteogenic differentiation induced by glucocorticoid.
