Enhancement of meniscal tearing damage repairing in the avascular zone using connective tissue growth factor (CT-GF) in the rabbit model
10.3969/j.issn.1003-0034.2013.08.017
- VernacularTitle:结缔组织生长因子促进半月板无血管区损伤愈合
- Author:
Wei HE
1
;
Ming LIU
;
Hong Zu QING
;
Wei GAO
;
Jie Yu LIU
;
Nan Xiao HUANG
Author Information
1. 解放军 305 医院骨科
- Keywords:
Connective tissue growth factors (CTGF);
Fibrochondrocyte;
Meniscus;
Vascular endothelial growth factors(VEGF);
Collagen typeⅠ;
Collagen typeⅡ
- From:
China Journal of Orthopaedics and Traumatology
2013;(8):681-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of connective tissue growth factors (CTGF) on secretion of extracellular matrix synthesis of meniscal fibrochondrocytes,expression of vascular endothelial growth factors (VEGF),and angiogenesis during the repair of meniscal tearing damage. Methods:Meniscal fibrochondrocytes were isolated from the inner-1/2 of rabbits'meniscus by collagenase enzymatic digestion,centrifugal separation,and treated with 100 ng/ml CTGF in vitro. Characteriza-tion of fibrochondrocytes was identified by flow cytometry analysising CD31 ,CD44,CD45 and CD105,and was further tested by typeⅡcollagen immunocytochemistry. Changes in gene expression of meniscal fibrochondrocytes were monitored by quanti-tative real-time polymerase chain reaction. In vitro ,the sections of the 3 mm of the longitudinal teared in the middle of the rab-bit's meniscus,and then the defects were dealed with simple suture,suture and implanting with PBS-fibrin glue,sutured and implanting with 1.5μg CTGF respectively. Expression and distribution of typeⅠandⅡcollagen and VEGF,the tearing healing were observed by fluorescence-immunohistochemisty analysis on the 1st week ,the 4th week and the 10th week. Results:Quantitative RT-PCR assays showed that typeⅠand typeⅡcollagen,and VEGF mRNA expression in the 100 ng/ml CTGF group had been remarkably enhanced than in the PBS group on the 14th day. Consistent with these effects in vitro ,fluores-cence-immunohistochemical analysis revealed that in the group implanted with CTGF-fibrin glue ,typeⅠcollagen,typeⅡcol-lagen and capillaries completely filled the defect on the 10th week postoperatively. In contrast ,only soft tissue repair occurred after the PBS-fibrin glue was implanted. Conclusion:CTGF can significantly promote extracellular matrix (Ⅰcollagen,Ⅱcol-lagen) of the meniscal avascular zone synthesis,and CTGF can greatly heighten the expression of VEGF activity at the same time in vitro,so that it can further enhance the repair of meniscal tearing damage in the avascular zone.
