Experimental Studies on Therapeutic Effect of HeiHuDan on the Rabbit Models of OA
10.3969/j.issn.1003-0034.2001.02.009
- VernacularTitle:黑虎丹治疗兔骨关节炎的实验研究
- Author:
Rong WAN
1
;
Qing-Ming YANG
;
Lian-Fu DENG
Author Information
1. Shanghai Institute of Traumatology and Orthopaedics
- From:
China Journal of Orthopaedics and Traumatology
2001;14(2):85-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of HeiHuDan（HHD） in the treatment of osteoarthritis（OA） and the proliferation and apoptosis of chondrocytes．Methods The anterior，posterior Cruciate Ligaments and medial menisci of the left knee in 36 adult New Zealand rabbits were sectioned to produce models of OA；the animals were then randomly divided into test-group and control-group．HHD 1 tab per day were given to the animals in the test group for 13 weeks after surgery．16th，20th，and 24th weeks after operation，the animals were sacraficed respectively and specimens were procured from the medial femoral condylar and the medial plateau of tibia for light microscopic，transmission electron microscopic and scanning electron microscopic study．Immunohistochemical method and in situ end labbelling method were used to detect the proliferation and apoptosis of chondrocytes in articular cartilage． Results The degenerative changes of cartilage in test-group had no significant difference from control’s，the apoptosis index and proliferative index of chondrocytes were less than control’s （P＜0.05） at 24 weeks postoperation．Conclusion The study indicated that HHD can decrease the apoptosis of chondrocytes in the late stage of OA and keep the balance of proliferation and apoptosis of OA chondrocytes．
