Antimicrobial susceptibility and molecular characterization of Streptococcus agalactiae in pregnant women with advanced maternal age in Tangshan City
10.12138/j.issn.1671-9638.20245195
- VernacularTitle:唐山地区高龄孕妇携带的无乳链球菌药物敏感性和分子特征研究
- Author:
Ze-Liang LIU
1
,
2
;
Xin-Yi GONG
;
Xiao HAN
;
Xue-Qi JIANG
;
Bei-Bei MIAO
;
Shuang MENG
;
Ai-Ying DONG
;
Juan LI
Author Information
1. 中国疾病预防控制中心传染病预防控制所细菌耐药控制室,传染病溯源预警与智能决策全国重点实验室,北京 102206
2. 华北理工大学附属医院微生物科,河北唐山 063000
- Keywords:
Streptococcus agalactiae;
antimicrobial susceptibility;
genome sequencing;
molecular type;
sero-type;
virulence gene
- From:
Chinese Journal of Infection Control
2024;23(10):1199-1205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the antimicrobial susceptibility,molecular types,serotypes,virulence factors and resistance mechanisms of Streptococcus agalactiae(S.agalactiae)isolated from pregnant women with ad-vanced maternal age in Tangshan City,and provide basic data for the treatment,prevention and control of S.aga-lactiae infection.Methods 42 strains of S.agalactiae isolated from pregnant women with advanced maternal age in North China University of Science and Technology Affiliated Hospital as well as Tangshan Maternal and Child Health Hospital were collected.Detection of antimicrobial susceptibility and whole genome sequencing of 13 antimi-crobial agents were performed.Results The percentage of tetracycline,erythromycin,levofloxacin,and chloram-phenicol concurrently resistant strains was 7.1％,35.7％of the strains presented multidrug resistance to erythro-mycin,clindamycin,and levofloxacin.The carriage rates of resistance genes ermB and tetM were 66.7％and 47.6％,respectively.29 strains(69.0％)exhibited mutations in both gyrA and parC fluoroquinolone resistance determi-nants.42 strains of S.agalactiae belonged to 4 serotypes,namely ⅠB(35.7％),Ⅲ(33.3％),Ⅴ(26.2％),andⅠA(4.8％);and 11 sequence types(STs),with the highest proportion being ST10(35.7％)and ST19(31.0％);as well as 6 clonal complexes(CCs),among which CC19(42.9％)and CC12(35.7％)had the highest proportion.All S.agalactiae carried virulence factor-encoding genes of cfb,cylE,and pavA.Conclusion The molecular types and serotypes of S.agalactiae carried by pregnant women with advanced maternal age in Tangshan City pre-sent polymorphism,with obvious multidrug resistance,and carry multiple types of drug resistance genes and viru-lence genes.
