Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
10.1007/s11684-017-0518-7
- Author:
Zhou BO
1
;
Xu HONGBIN
;
Xia MENG
;
Sun CHAOYANG
;
Li NA
;
Guo ENSONG
;
Guo LILI
;
Shan WANYING
;
Lu HAO
;
Wu YIFAN
;
Li YUAN
;
Yang DEGUI
;
Weng DANHUI
;
Meng LI
;
Hu JUNBO
;
Ma DING
;
Chen GANG
;
Li KEZHEN
Author Information
1. Cancer Biology Research Center
- Keywords:
ovarian cancer;
metastasis;
miR-9;
E-cadherin
- From:
Frontiers of Medicine
2017;11(2):214-222
- CountryChina
- Language:Chinese
-
Abstract:
MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers.Dysfunctional miR-9 expression remains ambiguous,and no consensus on the metastatic progression of ovarian cancer has been reached.In this study,results from the bioinformatics analysis show that the 3'-UTR of the E-cadherin mRNA was directly regulated by miR-9.Luciferase reporter assay results confirmed that miR-9 could directly target this 3'-UTR.miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRTPCR.Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780.qRT-PCR and Western blot were performed to detect the epithelial-mesenchymal transition-associated mRNA and proteins.Immunofluorescence technique was used to analyze the expression and subcellular localization of Ecadherin,N-cadherin,and vimentin.The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones.Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin).Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer,and these processes could be effectively inhibited via miR-9 inhibitor.Thus,our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.
