An analysis of genetic polymorphism and mutation rate of 36 Y-STR loci in Sichuan Han population
10.13618/j.issn.1001-5728.2024.05.009
- VernacularTitle:四川汉族人群36个Y-STR基因座的遗传多态性与突变率调查
- Author:
Shilin ZHANG
1
;
Wei WANG
;
Xiao ZHANG
;
Wenli SHI
;
Yanjie DING
;
Dongdong XU
;
Xiaohua ZHU
;
Bo JIN
Author Information
1. 川北医学院基础医学与法医学研究所,四川 南充 637000
- Keywords:
Forensic medicine;
Y-STR;
Gene diversity;
Mutation rate;
Sichuan han population
- From:
Chinese Journal of Forensic Medicine
2024;39(5):565-571,583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the genetic polymorphism and mutation rate of 36 Y-STR loci in Sichuan Han population and provide basic data for their forensic applications.Methods Blood samples were collected from 307 unrelated male individuals and 367 father-son pairs in Sichuan Han population using FTA blood sampling cards.The Y41SE-v1.2 kit was used to type 36 Y-STR loci,and the population genetic parameters and mutation rates of each locus were calculated.The genetic relationship between Sichuan Han population and other ethnic populations was analyzed in combination with 19 other populations in China.Results A total of 79 mutations were observed in 36 Y-STR loci in 367 father-son pairs of Sichuan Han population,with an average mutation rate of 6.00×10-3.There were 74(93.67%)one-step mutations and 5(6.33%)multi-step mutations.A total of 383 alleles were found at 36 Y-STR loci in 307 Sichuan Han males.The number of alleles at each locus ranged from 5(0.016 29)to 56(0.182 41),GD ranged from 0.100 02(DYS645)to 0.959 99(DYS385),and 306 haplotypes were found.The HD,DC and HMP values were 0.999 973,99.67%and 0.003 284,respectively.The results of genetic distance(Fst)analysis showed that the Sichuan Han population had the closest genetic distance with the Chongqing Han population(0.000 9)and the furthest genetic distance with the Xinjiang Mongolian group(0.032 0).The MDS based on genetic distance was basically consistent with the results of cluster analysis.Conclusion The 36 Y-STR loci have high genetic polymorphism in the Sichuan Han population.The study data can provide data support for the construction of the Y-STR database in this region,and also provide basic genetic information for forensic investigation and population genetics research.
