Fetal STR typing and paternity identification of early pregnancy aborted tissue based on next-generation sequencing technology
10.13618/j.issn.1001-5728.2024.05.004
- VernacularTitle:基于新一代测序技术进行孕早期流产组织胎儿STR分型及亲子鉴定
- Author:
Jin ZHANG
1
;
Kaihui LIU
;
Jinping HAO
;
Xueying YANG
;
Xingkun ZHANG
;
Wei PENG
;
Xiaoyu XU
;
Shan GAO
;
Jingjing CHANG
;
Bo LEI
;
Mengnan ZHANG
;
Qiujuan WANG
;
Ying ZHANG
Author Information
1. 公安部鉴定中心,北京 100038
- Keywords:
Forensic science;
Aborted tissue;
Next generation sequencing;
Short tandem repeats;
Mixed STR typing
- From:
Chinese Journal of Forensic Medicine
2024;39(5):539-545
- CountryChina
- Language:Chinese
-
Abstract:
Fetal STR typing of aborted tissue has long been a major problem in forensic DNA.Especially for the first trimester abortion tissue,it is difficult to isolate the embryonic components by histomorphological means,resulting in the inability to accurately obtain the STR typing of the fetus.The mixed STR typing results of mother and fetus can provide a key basis for the identification of suspects in cases of rape-induced pregnancy.In this study,next generation sequencing was used to successfully detect mixed STR typing of mother and suspected fetus or single STR typing of suspected fetus in 4 rape-induced early pregnancy abortion tissues.Combined with Y-STR and flank sequence information,it provides a more comprehensive and reliable genetic basis for the identification of suspects.
