Determination of blood nitrite and its stability by 1,8-Diaminonaphthalene derivatized GC-MS method

VernacularTitle:1,8-二氨基萘衍生化GC-MS法检测血液中亚硝酸盐及其稳定性
Author: Baihui CHEN ¹ ; Jing SUN ; Yunqian LI ; Guobin XIN
Author Information

1. 山西医科大学法医学院,山西晋中 030600
Keywords: Forensic poison analysis; Poisoning; GC-MS; Nitrite
From: Chinese Journal of Forensic Medicine 2024;39(4):462-466
CountryChina
Language:Chinese
Abstract: Objective To establish a gas chromatography-mass spectrometry(GC-MS)method for blood samples of death cases with nitrite poisoning and examine the stability of nitrite in blood,so as to provid a reference for inferring the concentration of nitrite in blood at the time of death and determining whether the death was caused by nitrite poisoning.Methods The 1,8-diaminonaphthalene(1,8-DAN)derivatized GC-MS method was used to detect the blood samples of people who died of nitrite poisoning;Blank blood was taken with nitrite standards,and the content was measured regularly.The stability of nitrite in blood was observed,and its concentration change trend was obtained.Results The concentration in blood of 11 cases of nitrite poisoning deaths ranged from 1.165 μg/mL to 351.551 μg/mL;With the increase of time,the concentration of nitrite in the labeled blood samples gradually decreased.Conclusion The detection method established in this research is easy to operate,has high accuracy and good precision.Nitrite is unstable in blood,it is recommended to detect it as soon as possible to prevent missed detection.