Detection of 13 Paralytic shellfish toxins in human whole blood by high-performance liquid chromatography-tandem mass spectrometry
10.13618/j.issn.1001-5728.2024.04.015
- VernacularTitle:UPLC-MS/MS检测全血中13种麻痹性贝类毒素
- Author:
Xiuqin MA
1
;
Qiang JIE
;
Yujing LUAN
;
Zeyu WANG
;
Fanglin WANG
Author Information
1. 山西医科大学,山西晋中 030600
- Keywords:
Forensic toxicological analysis;
Blood;
UPLC-MS/MS;
Paralytic shellfish toxins
- From:
Chinese Journal of Forensic Medicine
2024;39(4):458-461,466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the analysis method of 13 Paralytic shellfish toxins(PSTs)in human whole blood by high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods 13 kinds of PSTs were extracted from the whole blood using acetonitrile with 1%acetate(1︰3,v︰v)solution,and separated by UPLC BEH Amide chromatographic column(100mm×2.1mm,1.7μm).The samples were detected by an electrospray ionization source(ESI)and positive and negative ion multiple reaction monitoring(MRM)mode,and quantified by matrix matching curve external standard method.Results The results showed that 13 kinds of PSTs in blood samples were linear well in the ranges of 1～100 ng/mL,with the correlation coefficient(r2)>0.995.The limit of detection(LODs)of the method were 0.5～2 ng/mL,the limit of quantitation(LOQs)were 1～4 ng/mL,and the recoveries of the method were 65.55%～114.12%.Conclusion The method is highly sensitive,reproducible,and can qualify and quantify thirteen toxins at the same time,which is suitable for the rapid detection of PSTs in whole blood samples.
