Study on metabolism of phenethylamines new psychoactive substances 2C-B-FLY and 25B-NBOH in vitro
10.13618/j.issn.1001-5728.2024.04.013
- VernacularTitle:苯乙胺类新精神活性物质2C-B-FLY和25B-NBOH的体外代谢研究
- Author:
Yan SHI
1
;
Wenya ZHAI
;
Xin WANG
;
Shuo YANG
;
Hejian WU
Author Information
1. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台司法部司法鉴定重点实验室,上海 200063
- Keywords:
New psychoactive substance;
2C-B-FLY;
25B-NBOH;
Metabolites in vitro;
Metabolic pathway
- From:
Chinese Journal of Forensic Medicine
2024;39(4):444-449,456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro metabolites and metabolic pathways of phenethylamine new psychoactive substances 2C-B-FLY and 25B-NBOH in human liver microsomes.Methods The human liver microsome incubation model was established,and the samples were centrifuged,blow-dried and re-dissolved after 3 h of co-incubation in a water bath at 37℃.The samples were detected by ultra-high performance liquid chromatography Q Exactive mass spectrometry in ESI+mode,and the parent drug and its metabolites were examined by applying Full scan-ddMS2 data-dependent acquisition mode.Results After in vitro incubation with human liver microsomes,2C-B-FLY was metabolised to produce a total of four phase I metabolites and one acetylated phase II metabolite,which underwent metabolic reactions such as hydroxylation,debromination,and N-acetylation.25B-NBOH was metabolised to produce a total of eight phase I metabolites and two glucuronidated phase II metabolites,which comprised the major biotransformation reactions of O-demethylation,hydroxylation,amine dehydrogenation,N-dealkylation and glucuronidation.Conclusion This study describes the metabolites and metabolic pathways of 2C-B-FLY and 25B-NBOH in a human liver microsomal incubation system,providing a scientific basis for the in vivo detection of new psychoactive substance of the phenethylamine group.
