Preliminary exploration of distinguishing samples from different parts of human body by microbial testing
10.13618/j.issn.1001-5728.2024.04.011
- VernacularTitle:利用微生物检验区分人体不同部位样本初探
- Author:
Zhen PENG
1
;
Bo YANG
;
Fan YANG
;
Meiqing YUAN
;
Xingchun ZHAO
Author Information
1. 山西医科大学法医学院,山西太原 030001
- Keywords:
Microbiome;
Biological traces;
Random forest model;
Trace to the source
- From:
Chinese Journal of Forensic Medicine
2024;39(4):434-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of tracing the source of human biological samples by microbiome,and provide new ideas for case investigation.Methods Biological samples from buccal mucosa,foot arch,armpit and other parts of 10 volunteers were collected for three consecutive months.The microbial community structure of the samples was confirmed by using the 16SrRNA gene sequence information.And the microbial community diversity analysis and random forest classification prediction model were carried out.Results There were significant differences in the microbial community structure of the three parts of human body.In this study,a prediction model of random forest classification with an accuracy of more than 90%was successfully constructed.Conclusion In this study,a classification and prediction model based on the microbiome information of human biological samples was constructed to judge the source location,which broadened the forensic application scenarios of human microorganisms.
