Research progress on environmental DNA detection and geographical origin inference in forensic science
10.13618/j.issn.1001-5728.2024.03.018
- VernacularTitle:环境DNA检验及地域来源推断研究进展
- Author:
Qi YANG
1
,
2
;
Kelai KANG
;
Hongcheng MEI
;
Jiajin PENG
;
Jiahui YUAN
;
Yaosen FENG
;
Jian YE
;
Anquan JI
;
Le WANG
Author Information
1. 法医遗传学公安部重点实验室,公安部鉴定中心,北京 100038
2. 中国人民公安大学,北京 100038
- Keywords:
Forensic biological evidence;
Environmental DNA;
Microbiome;
Next-generation sequencing;
Geographical origin inference
- From:
Chinese Journal of Forensic Medicine
2024;39(3):349-356
- CountryChina
- Language:Chinese
-
Abstract:
The geographical origin of forensic evidence provides important information for crime investigation and solving cases,and is one of the key elements of criminal cases.Previous studies have shown significant differences in the distribution of microorganisms in different regions.Detecting environmental DNA samples and inferring the geographical and spatial sources can provide clues and evidence for case handling.However,due to the diversity of criminal environments and the trace amount of frequently encountered exhibits,stable and reliable technical methods for inferring geographical origin from environmental DNA are not yet available.This article summarizes the sample collection and DNA extraction methods for four types of environmental samples:dust,soil,water,and air.It compares the differences between amplicon sequencing and metagenomic sequencing in studying environmental biological populations,outlines the full process of high-throughput sequencing-based data analysis,and focuses on reviewing the research progress in inferring geographical sources of environmental samples based on bacteria,fungi,and other eukaryotes,to provide references for establishing sequencing and analysis methods for environmental DNA in forensic DNA laboratories and exploring environmental DNA information for forensic applications.
