Progress in single cell isolation techniques in forensic science
10.13618/j.issn.1001-5728.2024.03.017
- VernacularTitle:单细胞分离技术在法医学中的应用研究进展
- Author:
Kesheng SUN
1
;
Haoyu GU
;
Feng SONG
;
Yingchun DONG
;
Haibo LUO
Author Information
1. 广东太太法医物证司法鉴定所,深圳 518057
- Keywords:
Single cell isolation;
Forensic science;
Mixed stain;
Manipulation
- From:
Chinese Journal of Forensic Medicine
2024;39(3):339-348
- CountryChina
- Language:Chinese
-
Abstract:
Forensic examination materials are often plagued by trace amounts,mixes,and other factors.Single-cell isolation technology can solve these forensic problems to some extent by studying each cell individually to obtain comprehensive and reliable information.There are many single cell isolation techniques available in research reports,such as flow cytometry,laser capture microdissection,etc.This review will summarize the most common single cell isolation techniques used by researchers today,and summarize the application of various techniques in forensic science,summarize the selection strategies for single-cell isolation techniques in different scenarios based on cost,degree of automation,yield,cell damage rate,and the availability of relevant forensic platforms,and finally explore the forensic application prospects of single-cell isolation techniques.In general,single cell isolation can be applied to multiple fields such as mixed stain examination,post-mortem time inference,pre-and post-mortem injury determination,forensic toxicology analysis,forensic microbiology and forensic anthropology.The development of single cell isolation technology is of great value to the application of forensic medicine,and will provide a new way of deciphering difficult examination materials.
