Δ9-Tetrahydrocannabinol and other four components in industrial hemp and its processed products were detected by UPLC and UPLC-MS/MS methods
10.13618/j.issn.1001-5728.2024.03.016
- VernacularTitle:UPLC和UPLC-MS/MS法检验工业大麻及其加工产品中Δ9-四氢大麻酚
- Author:
Yuexin LIU
1
;
Wu WEN
;
Qun JIA
;
Xinyi LI
;
Zhiqiang LU
;
Fazhen YANG
;
Hong LI
Author Information
1. 昆明医科大学法医学院,云南 昆明 650500
- Keywords:
Forensic toxicology analysis;
Δ9-THC;
Industrial marijuana;
LC;
LC-MS
- From:
Chinese Journal of Forensic Medicine
2024;39(3):335-338,367
- CountryChina
- Language:Chinese
-
Abstract:
Objective The Δ9-tetrahydrocannabinol in the flowers and leaves of industrial hemp plants and hemp processing products was extracted and separated,to establish a liquid chromatography-mass spectrometry(LC-MS)qualitative and quantitative test method and a liquid chromatography(LC)quantitative test method for Δ9-THC.Methods The samples were dried naturally and ground,extracted with methanol and analyzed.The ACQUITY UPLC ? BEH C18 column was used.The liquid chromatography was performed on a gradient elution with water and acetonitrile.The detection wavelength was 215 nm and the bandwidth was 4.8 nm.Liquid chromatography-mass spectrometry was performed with 0.1%formic acid water and 0.1%formic acid acetonitrile for gradient elution.Electrospray ion source,scanning mode:positive ion mode,multiple characteristic ion pairs for qualitative analysis,and externalstandard curve method for quantitative analysis.Results The linearity of Δ9-THC in the range of 0.001～0.1 mg/mL in liquid chromatography and 5～100 ng/mL in liquid chromatography-mass spectrometry was good,R2 were greater than 0.999.The relative error of the measured values of the liquid chromatography and liquid chromatography-mass spectrometry test methods was within±2%,and the precision was less than 2%.Conclusion The method established in this paper can be applied to the detection of Δ9-THC in industrial cannabis plants and cannabis processing products,which can provide technical support for the detection of industrial cannabis plants and cannabis processing products in the laboratory.
