Research on GC-MS/MS qualitative result evaluation of six common drugs in blood
10.13618/j.issn.1001-5728.2024.03.015
- VernacularTitle:血液中6种常见毒品的GC-MS/MS定性结果评价探究
- Author:
Baihui CHEN
1
;
Guobin XIN
;
Tao MIN
;
Jing SUN
;
Shihao ZHONG
;
Yuanfeng WANG
Author Information
1. 山西医科大学法医学院,山西 晋中 030600
- Keywords:
Forensic toxicology analysis;
Retention time;
Ion ratios;
GC-MS/MS;
Drugs
- From:
Chinese Journal of Forensic Medicine
2024;39(3):328-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate a gas chromatography-tandem mass spectrometry(GC-MS/MS)method for the detection of six common drugs(methamphetamine,meperidine,caffeine,codeine,cocaine and ketamine)in blood,and to improve the determination basis of results.Methods The above six drugs were added into the blank blood,and GC-MS/MS was used for detection after ether extraction.The collection,quantification and confirmation were carried out under the mode of multi-reaction monitoring(MRM).The qualitative results of the above six drugs were evaluated based on the maximum allowable deviation of the retention time and relative ion abundance ratio in the qualitative results of GC-MS/MS.Results There was a good linear relationship between the six common drugs,among which ketamine and caffeine had the lowest detection limit(0.01 μg/mL),methamphetamine had the highest detection limit(0.5 μg/mL).The retention time(RT)and relative retention time(RRT)of the target substance were stable under the six supplemental levels,and the absolute deviation(ΔRTabsolute)of RT was within±0.025 min.The absolute deviation of RRT(ΔRRTabsolute)was within±0.004.The relative ion abundance ratio absolute deviation(ΔIabsolute)is±20%,and the relative ion abundance ratio relative deviation(ΔIrelative)is±50%.Conclusion This study clarified the reference range for qualitative determination of six common drugs in blood matrix detected by GC-MS/MS,and effectively supplemented the qualitative determination indicators of existing instrumental analysis methods.
