Maternal high-fat diet during pregnancy promotes aortic endothelial to mesenchymal transition in offspring
10.20039/j.cnki.1007-3949.2024.11.003
- VernacularTitle:母亲孕期高脂饮食促进子代主动脉血管内皮间充质转变
- Author:
Hui HE
1
;
Yuan ZHU
;
Doudou DONG
;
Meng DING
;
Tianyu JIANG
;
Xiaochuan MO
;
Zhuting WANG
;
Hailong OU
Author Information
1. 贵州医科大学基础医学院生物化学与分子生物学教研室,贵州省贵阳市 561113
- Keywords:
high-fat diet;
endothelial to mesenchymal transition;
Kruppel-like factor 2;
mice;
maternal pregnancy;
offspring
- From:
Chinese Journal of Arteriosclerosis
2024;32(11):939-946
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of maternal high-fat diet during pregnancy on endothelial to mesenchymal transition of aortic vessels in adult offspring.Methods The pregnant mice were randomly divided into normal diet group and high-fat diet group,and the offspring mice were fed normally for 16 weeks after the mother gave birth.Western blot and RT-qPCR were used to detect the expression and transcription of related proteins,and immunofluorescence and im-munohistochemical staining were used for pathological analysis.Results Compared with the offspring of maternal nor-mal diet during pregnancy,the expressions of vascular inflammatory factors,macrophage infiltration,monocyte-endothelium adhesion were significantly increased in the offspring of maternal high-fat diet(OHF)during pregnancy(P＜0.05).Vas-cular endothelial nitric oxide synthase(eNOS)activity,nitric oxide(NO)level were dramatically reduced(P＜0.05).Immunofluorescence results showed reduced endothelial cell marker CD31 and increased mesenchymal marker α-smooth muscle actin(α-SMA)in OHF.Western blot analysis further confirmed the results,which showed that maternal high fat diet reduced vascular endothelial-cadherin(VE-cadherin)and CD31 and increased α-SMA and Vimentin in the offspring(P＜0.05).The maternal high fat diet increased the extracellular matrix protein disposition and transforming growth factor beta(TGF-β)/Smad signaling in endothelium(P＜0.05).Moreover,the maternal high fat diet reduced Kruppel-like factor 2(KLF2)expression by 76％in mRNA level and 59％in protein level(P＜0.05).Conclusion Maternal high-fat diet during pregnancy lead to a transition of endothelial to mesenchyme in the offspring aorta.The results provide a clue for prevention of vascular disease in early stage.
