Adipose derived mesenchymal stem cell exosomes inhibit adverse ventricular remode-ling after myocardial infarction by regulating autophagy and NLRP3 inflammasomes balance of cardiac fibroblasts
10.20039/j.cnki.1007-3949.2024.08.002
- VernacularTitle:脂肪间充质干细胞外泌体调控心脏成纤维细胞自噬和NLRP3炎症小体平衡抑制心肌梗死后不良心室重塑
- Author:
Jianjun WANG
1
;
Jing LI
;
Xuming MA
;
Zhaofei WAN
;
Bin ZHU
;
Yaping LIU
;
Xiangqian GUO
;
Jiping PAN
;
Yan FAN
Author Information
1. 兰州市第一人民医院骨科,甘肃省兰州市 730050
- Keywords:
adipose derived mesenchymal stem cell;
exosomes;
autophagy related 16 like protein 1;
autophagy related protein 7;
NOD-like receptor protein 3 inflammasomes;
myocardial fibrosis;
ventricular remodeling;
myocardial infarction
- From:
Chinese Journal of Arteriosclerosis
2024;32(8):654-662
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibition role and mechanism of adipose derived mesenchymal stem cell(ADMSC)exosomes(Exo)on adverse ventricular remodeling after myocardial infarction(MI).Methods The chan-ges of autophagy and inflammasomes phenotype of cardiac fibroblasts after H2O2 treatment were observed.MI rats were in-jected with an equal volume of normal saline,adipose derived mesenchymal stem cell exosomes(MSC-Exo)or fibroblast exosomes(MEF-Exo)via a tail vein.The expression of autophagy related 16 like protein 1(ATG16L1),autophagy re-lated protein 7(ATG7)and NOD-like receptor protein 3(NLRP3),inflammatory response,the degree of myocardial fi-brosis,and the cardiac function were observed in different groups.Results After treatment with H2O2 on cardiac fi-broblasts,the expressions of ATG16L1 and ATG7 were significantly decreased(P＜0.001),NLRP3 was significantly in-creased(P＜0.001),and the levels of inflammatory cytokines interleukin-1β(IL-1β)and IL-18 were significantly elevated(P＜0.001).After MI rats were intervened with MSC-Exo,the expressions of autophagy related proteins ATG16L1 and ATG7 were significantly up-regulated(P＜0.001),NLRP3 was significantly down-regulated(P＜0.001),serum IL-1β and IL-18 levels were significantly decreased(P＜0.001),fibrosis-related proteins collagen Ⅰ and Ⅲ were significantly reduced(P＜0.001),myocardial fibrosis was significantly relieved(P＜0.001),and cardiac function was sig-nificantly improved(P＜0.001).Conclusion Adipose derived MSC-Exo play a role in inhibiting adverse ventricular remodeling after MI by regulating the balance of autophagy and NLRP3 inflammasomes.
