Development of an Integrated Reporting System for Verifying Hemolysis, Icterus, and Lipemia in Clinical Chemistry Results.
10.3343/alm.2014.34.4.307
- Author:
Dong Hoon SHIN
1
;
Juwon KIM
;
Young UH
;
Se Il LEE
;
Dong Min SEO
;
Kab Seung KIM
;
Jae Yun JANG
;
Man Hee LEE
;
Kwang Ro YOON
;
Kap Jun YOON
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea.
- Publication Type:Original Article
- Keywords:
Hemolysis;
Icterus;
Lipemia;
Preanalytical interference
- MeSH:
Blood Chemical Analysis/instrumentation/*methods/standards;
Female;
Hemoglobins/analysis;
*Hemolysis;
Humans;
Hyperlipidemias/metabolism/*pathology;
Jaundice/metabolism/*pathology;
Male;
Quality Control;
Reproducibility of Results
- From:Annals of Laboratory Medicine
2014;34(4):307-312
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Hemolysis, icterus, and lipemia (HIL) cause preanalytical interference and vary unpredictably with different analytical equipments and measurement methods. We developed an integrated reporting system for verifying HIL status in order to identify the extent of interference by HIL on clinical chemistry results. METHODS: HIL interference data from 30 chemical analytes were provided by the manufacturers and were used to generate a table of clinically relevant interference values that indicated the extent of bias at specific index values (alert index values). The HIL results generated by the Vista 1500 system (Siemens Healthcare Diagnostics, USA), Advia 2400 system (Siemens Healthcare Diagnostics), and Modular DPE system (Roche Diagnostics, Switzerland) were analyzed and displayed on physicians' personal computers. RESULTS: Analytes 11 and 29 among the 30 chemical analytes were affected by interference due to hemolysis, when measured using the Vista and Modular systems, respectively. The hemolysis alert indices for the Vista and Modular systems were 0.1-25.8% and 0.1-64.7%, respectively. The alert indices for icterus and lipemia were <1.4% and 0.7% in the Vista system and 0.7% and 1.0% in the Modular system, respectively. CONCLUSIONS: The HIL alert index values for chemical analytes varied depending on the chemistry analyzer. This integrated HIL reporting system provides an effective screening tool for verifying specimen quality with regard to HIL and simplifies the laboratory workflow.
