Genetic characteristics of human infection with Brucella melitensis in Nanjing from 2017 to 2022
10.3760/cma.j.cn231583-20240104-00006
- VernacularTitle:2017 - 2022年南京市人感染羊种布鲁氏菌基因特征研究
- Author:
Weixiang WANG
1
;
Lu ZHOU
;
Jingjing SU
;
Nan ZHANG
;
Jie HONG
;
Weizhong ZHOU
;
Changjun BAO
;
Zhongming TAN
Author Information
1. 南京市疾病预防控制中心急性传染病防制科，南京 210000
- Keywords:
Brucella;
Multiple-locus variable number tandem repeat analysis;
Multilocus sequence typing;
Single nucleotide polymorphism
- From:
Chinese Journal of Endemiology
2024;43(10):775-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the distribution of species type, biotype and genotype of human Brucella isolated and identified in Nanjing. Methods:A total of 89 strains of human Brucella were collected from microbiology laboratories of three sentinel hospitals in Nanjing from 2017 to 2022. The species type was identified using biological methods and Brucella nucleic acid detection (BCSP31-PCR and AMOS-PCR). Further biotyping of Brucella melitensis isolates was conducted by serological results of A and M factors. Meanwhile, genotype analysis was performed using multiple-locus variable number tandem repeat analysis (MLVA), multilocus sequence typing (MLST) and single nucleotide polymorphism (SNP). Results:From 2017 to 2022, 89 strains of Brucella isolated and identified in Nanjing were all Brucella melitensis. Among them, Brucella melitensis biotype 3 accounted for 82.02% (73/89), and biotype 1 accounted for 17.98% (16/89). MLVA typing showed that 89 strains of Brucella melitensis belong to the "Eastern Mediterranean" cluster and could be divided into 50 MLVA genotypes; among which panel 1 had 3 genotypes, namely Type 42 (84.27%, 75/89), Type 63 (8.99%, 8/89) and Type 43 (6.74%, 6/89). The results of MLST-9 and MLST-21 were both ST8, and the core genome multilocus sequence typing (cgMLST) classified 89 strains into 11 genotypes. SNP analysis revealed a total of 4 013 SNP loci, with SNPs ranging from 0 to 409 across different strains, involving 59 SNP genotypes. Conclusions:The human Brucella strains isolated and identified in Nanjing are all Brucella melitensis, mainly biotype 3. The MLVA cluster is the "Eastern Mediterranean" cluster. The traditional MLST-9 and MLST-21 typing results are all ST8 type, while cgMLST divides all the strains into 11 genotypes with higher resolution.
