A method for detecting urine iodine by antimony cerium catalytic spectrophotometry
10.3760/cma.j.cn231583-20240122-00023
- VernacularTitle:锑铈催化分光光度法检测尿碘的方法研究
- Author:
Shudong XU
1
;
Ji'an XIE
;
Gang DING
;
Xian XU
;
Weidong LI
Author Information
1. 安徽省疾病预防控制中心理化检验室，合肥 230601
- Keywords:
Urine;
Iodine;
Antimony cerium catalysis;
Spectrophotometry
- From:
Chinese Journal of Endemiology
2024;43(9):750-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a new method for antimony cerium catalytic spectrophotometric detection (referred to as the new method) of urinary iodine.Methods:By utilizing the efficient catalytic ability of iodine for the oxidation-reduction reaction of antimony and cerium, a new method was applied to conduct linear relationship fitting curve, detection limit, pecision, accuracy and other experiments within the range of 0 - 300 μg/L urine iodine mass concentration. The method was compared with the Arsenic Cerium Catalytic Spectrometric Method (WS/T 107.1-2016) recommended national industry standard.Results:In the range of 0 - 300 μg/L urine iodine mass concentration, the correlation coefficients│ r│ of the fitted curves were > 0.999 0, and the qualitative detection limit was 7.96 μg/L (with a sampling volume of 0.25 ml). Four actual urine samples with different iodine mass concentrations were selected for precision experiments, and the relative standard deviation ( RSD) of the detection precision ranged from 0.96% to 2.87%, all < 5%. The actual urine sample spiked recovery range from 99.62% to 103.88%, with an overall average recovery rate of 101.75%. Two methods were used to detect 30 urine samples, and the difference in results was not statistically significant ( t = 0.68, P = 0.501). Conclusions:The new method has high reaction sensitivity and accuracy, avoiding the use of highly toxic substance arsenic trioxide. The antimony reagent used is safe, and the environmental pollution caused by waste liquid discharge is small. The detection steps are similar to Arsenic Cerium Catalytic Spectrometric Method, the laboratory urine iodine detection personnel do not need special training to operate and use it, which is suitable for the application and promotion of urine iodine detection in grassroots iodine deficiency disorders monitoring work.
