Detection of resistance and knock down resistance gene mutation of Culex pipiens pallens to pyrethroid insecticides in Hangzhou City
10.3760/cma.j.cn231583-20231008-00077
- VernacularTitle:杭州市淡色库蚊对拟除虫菊酯类杀虫剂的抗药性和击倒抗性基因突变检测
- Author:
Binbin JIN
1
;
Lingya WEI
;
Tianxiao DUAN
;
Bing WANG
;
Hui JIN
Author Information
1. 杭州市疾病预防控制中心（杭州市卫生监督所）消毒与病媒生物防制所，杭州 310021
- Keywords:
Culex pipiens pallens;
Pyrethroid insecticides;
Resistance;
Knock down resistance gene
- From:
Chinese Journal of Endemiology
2024;43(7):523-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the resistance of Culex pipiens pallens to pyrethroid insecticides in Hangzhou City, and explore the mutation of knock down resistance (kdr) gene of Culex pipiens pallens, providing scientific basis for control of Culex pipiens pallens in the region. Methods:In September 2022, larvae and pupae of Culex pipiens pallens were collected from various locations at national monitoring sites in Shangcheng District and Gongshu District of Hangzhou City. They were bred in the laboratory and their resistance to three commonly used pyrethroid insecticides (permethrin, deltamethrin and beta-cypermethrin) was determined using the adult mosquito contact tube method and larval immersion method recommended by the World Health Organization. The genomic DNA was extracted from individual adult mosquito, and kdr gene mutation was detected via PCR amplification and Sanger sequencing. Results:The 24-hour mortality rates of adult mosquitoes of Culex pipiens pallens exposed to 0.25% permethrin, 0.025% deltamethrin, and 0.025% beta-cypermethrin were 20.00% (15/75), 17.33% (13/75), and 18.67% (14/75), respectively, indicating resistance. The resistance ratios of Culex pipiens pallens larvae to permethrin, deltamethrin, and beta-cypermethrin were 27.08, 341.00, and 15.88, respectively. Among 183 adult mosquitoes that survived under the diagnostic doses of pyrethroid insecticides, 180 mosquitoes (98.36%) had mutation at the L1014 site of the kdr gene. Among 42 adult mosquitoes that died under the diagnostic doses of pyrethroid insecticides, 5 mosquitoes (11.90%) had L1014 mutation in the kdr gene. Conclusions:Culex pipiens pallens in Hangzhou City has developed varying degrees of resistance to permethrin, deltamethrin and beta-cypermethrin, with a high frequency of kdr gene mutation in resistant mosquitoes. We should strengthen the monitoring of the resistance of Culex pipiens pallens in Hangzhou City and use chemical insecticides reasonably.
