Identification and homology analysis of Brucella in familial clustered infections
10.3760/cma.j.cn231583-20230317-00060
- VernacularTitle:家庭聚集性感染布鲁氏菌的鉴定及同源性分析
- Author:
Danting JIN
1
;
Jin YANG
;
Zhongming TAN
;
Xinzhe WU
;
Chunlei ZUO
;
Xiuzhen ZOU
;
Haifeng MAO
Author Information
1. 江苏省连云港市第一人民医院检验科，连云港 222002
- Keywords:
Brucella;
Multilocus sequence typing;
Identification
- From:
Chinese Journal of Endemiology
2024;43(5):350-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify species type and analyze the homology of Brucella in a family cluster infection. Methods:Two patients with brucellosis from the same family who were treated at the First People's Hospital of Lianyungang City, Jiangsu Province in May 2022 were selected as the research subjects. Brucella strains (H4LYG01 and H2LYG02) were isolated through blood culture. The isolated strains were identified for species type and homology analysis using a fully automated microbial mass spectrometry detection system. Molecular typing of the isolated strains was performed using multiple locus variable-number tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). Results:H4LYG01 and H2LYG02 were both Brucella Maltese, and the credibility scores were 9.745 and 9.627, respectively, and the homology was 100%. The MLVA results showed that the gene loci of H4LYG01 and H2LYG02 were completely identical, with the same genotype. The MLST results showed that the sequence type (ST) of H4LYG01 and H2LYG02 were both ST8 types. Conclusion:The two isolated strains of Brucella from a family with clustered infections are both Brucella Maltese and from the same source.
